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| 題名: | A shRNA functional screen reveals Nme6 and Nme7 are crucial for embryonic stem cell renewal |
| 作者: | 馬念涵;Wang, Chia-Hui;Ma, Nianhan;Lin, Yu-Tsen;Wu, Cheng-Chung;Hsiao, Michael;Lu, Frank Leigh;Yu, Ching-Chia;Chen, Shao-Yin;Lu, Jean |
| 貢獻者: | 生醫理工學院生醫科學與工程學系 |
| 關鍵詞: | Animals;Cell Differentiation;Cell Line;Embryoid body;Embryonic stem cells;Embryonic Stem Cells - cytology;Embryonic Stem Cells - metabolism;Gene Expression;Gene Expression Profiling;Gene Knockout Techniques;High-Throughput Screening Assays;Kinases;Leukemia;Mice;Nucleoside-Diphosphate Kinase - genetics;Nucleoside-Diphosphate Kinase - metabolism;Pluripotency;Pluripotent Stem Cells - cytology;Pluripotent Stem Cells - metabolism;RNA, Small Interfering - genetics;Stem cells;Teratoma |
| 日期: | 2012-10-01 |
| 上傳時間: | 2026-04-23 11:14:54 (UTC+8) |
| 出版者: | Wiley-Blackwell;Hoboken: Wiley Subscription Services, Inc., A Wiley Company |
| 摘要: | 摘要： AbstractIn contrast to the somatic cells, embryonic stem cells (ESCs) are characterized by its immortalization ability, pluripotency, and oncogenicity. Revealing the underlying mechanism of ESC characteristics is important for the application of ESCs in clinical medicine. We performed systematic functional screen in mouse ESCs with 4,801 shRNAs that target 929 kinases and phosphatases. One hundred and thirty-two candidate genes that regulate both ESC expansion and stem cell marker expression were identified. Twenty-seven out of the 132 genes were regarded as most important since knockdown of each gene induces morphological changes from undifferentiated to differentiated state. Among the 27 genes, we chose nonmetastatic cell 6 (Nme6, also named as Nm23-H6) and nonmetastatic cell 7 (Nme7, also designated as Nm23-H7) to study first. Nme6 and Nme7 both belong to the members of nucleoside diphosphate kinase family. We demonstrate that Nme6 and Nme7 are important for the regulation of Oct4, Nanog, Klf4, c-Myc, telomerase, Dnmt3B, Sox2, and ERas expression. Either knockdown of Nme6 or Nme7 reduces the formation of embryoid body (EB) and teratoma. The overexpression of either Nme6 or Nme7 can rescue the stem cell marker expression and the EB formation in the absence of leukemia inhibiting factor. This implies the importance of Nme6 and Nme7 in ESC renewal. This finding not only pinpoints Nme6 or Nme7 can regulate several critical regulators in ESC renewal but also increases our understanding of the ESC renewal and oncogenesis.
其他題名： STEM CELLS
出版者： Hoboken: Wiley Subscription Services, Inc., A Wiley Company
出版日期： 2012-10
出處： Stem Cells, 2012-10, Vol.30 (10), p.2199-2211
版權： Copyright © 2012 AlphaMed Press
版權： Copyright © 2012 AlphaMed Press.
識別號： ISSN: 1066-5099
識別號： ISSN: 1549-4918
識別號： EISSN: 1549-4918
識別號： DOI: 10.1002/stem.1203
識別號： PMID: 22899353 |
| 顯示於類別: | [生醫科學與工程學系] 期刊論文
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