Aurora-A signaling is activated in advanced stage of squamous cell carcinoma of head and neck cancer and requires osteopontin to stimulate invasive behavior

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題名:	Aurora-A signaling is activated in advanced stage of squamous cell carcinoma of head and neck cancer and requires osteopontin to stimulate invasive behavior
作者:	蘇立仁;Chien, Chih-Yen;Tsai, Hsin-Ting;Su, Li-Jen;Chuang, Hui-Ching;Shiu, Li-Yen;Huang, Chao-Cheng;Fang, Fu-Min;Yu, Chun-Chieh;Su, Huei-Ting;Chen, Chang-Han
貢獻者:	生醫理工學院生醫科學與工程學系
關鍵詞:	Adult;Aged;Aged, 80 and over;Aurora Kinase A - metabolism;Blotting, Western;Carcinoma, Squamous Cell - metabolism;Carcinoma, Squamous Cell - pathology;Female;Head and Neck Neoplasms - metabolism;Head and Neck Neoplasms - pathology;Humans;Immunohistochemistry;Kaplan-Meier Estimate;Male;MAP Kinase Signaling System - physiology;Middle Aged;Neoplasm Invasiveness - physiopathology;Oligonucleotide Array Sequence Analysis;Osteopontin - metabolism;Proportional Hazards Models;Real-Time Polymerase Chain Reaction;Research Paper;Reverse Transcriptase Polymerase Chain Reaction;Signal Transduction - physiology;Squamous Cell Carcinoma of Head and Neck;Transfection
日期:	2014-01-01
上傳時間:	2026-04-23 11:15:12 (UTC+8)
出版者:	Impact Journals LLC;United States: Impact Journals LLC
摘要:	摘要： The clinical significances, cellular effects, and molecular mechanisms by which Aurora-A mediate its invasive effects in HNSCC are still unclear. Here, we found that Aurora-A expression is significantly higher in tumor tissues on 14-microarray of HNSCC in Oncomine-databases. The activity of Aurora-A was not only found in HNSCC specimens, but also significantly correlated with advanced-T-classification, positive-N-classification, TNM-stage and the poor 5-year survival rate. HNSCC-microarray profile showed that osteopontin and Aurora-A exhibited positive correlation. Stimulation of HNC cells with osteopontin results in an increase in Aurora-A expression where localized at the centrosome. Functionally, Aurora-A had the abilities to stimulate cell motility in HNC cells through increase ERK1/2 activity under osteopontin stimulation. Conversely, depletion of Aurora-A expression by siRNAs suppressed ERK1/2 activity as well as inhibition of cell invasiveness. Treatment with anti-CD44 antibodies in HNC cells not only caused a decrease of mRNA/protein of Aurora-A and ERK1/2 activity upon osteopontin stimulation, but also affected the abilities of Aurora-A-elicited cell motility. Finally, immunohistochemical/Western-blotting analysis of human aggressive HNSCC specimens showed a significant positively correlation between osteopontin-Aurora-A and ERK1/2. These findings suggest that Aurora-A is not only an important prognostic factor but also a new therapeutic target in the osteopontin/CD44/ERK pathway for HNSCC treatment. 其他題名： Oncotarget 出版者： United States: Impact Journals LLC 出版日期： 2014-04-30 出處： Oncotarget, 2014-04, Vol.5 (8), p.2243-2262 版權： Copyright: © 2014 Chien et al. 2014 識別號： ISSN: 1949-2553 識別號： EISSN: 1949-2553 識別號： DOI: 10.18632/oncotarget.1896 識別號： PMID: 24810160
顯示於類別:	[生醫科學與工程學系] 期刊論文

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