Chemopreventive and chemotherapeutic effects of dietary supplementation of vitamin D on cholangiocarcinoma in a Chemical-Induced animal model

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Title:	Chemopreventive and chemotherapeutic effects of dietary supplementation of vitamin D on cholangiocarcinoma in a Chemical-Induced animal model
Authors:	蘇立仁;Chiang, Kun-Chun;Yeh, Chun-Nan;Lin, Kun-Ju;Su, Li-Jen;Yen, Tzu-Chen;Pang, Jong-Hwei S.;Kittaka, Atsushi;Sun, Chi-Chin;Chen, Miin-Fu;Jan, Yi-Yin;Chen, Tai C.;Juang, Horng-Heng;Yeh, Ta-Sen
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Acute-Phase Proteins;Animals;Bile Duct Neoplasms;Bile Ducts, Intrahepatic;Body Weight;Calcium;Cell Growth Processes;Cell Line, Tumor;Cell Movement;Chemoprevention;Cholangiocarcinoma;Dietary Supplements;Disease Models, Animal;Disease Progression;Down-Regulation;Gene Expression Profiling;Humans;Lipocalin-2;Lipocalins;Male;Positron-Emission Tomography;Proto-Oncogene Proteins;Rats;Rats, Sprague-Dawley;Research Paper;Vitamin D
Date:	2014-01-01
Issue Date:	2026-04-23 11:15:19 (UTC+8)
Publisher:	Impact Journals LLC;Impact Journals, LLC
Abstract:	摘要： Intrahepatic cholangiocarcinoma (ICC) is an aggressive cancer. Vitamin D, a pro-hormone, is getting popular due to its hormone-like functions after converted to its active form, 1α,25(OH)2D3. Here, we show that dietary supplementation with 6 IU/g of vitamin D greatly suppressed ICC initiation and progression without apparent toxicity in a chemically induced rat model. Microarray analysis of rat ICC tissues showed vitamin D supplementation modulated the expressions of several unique genes, including lipocalin 2 (Lcn2), confirmed by RT-qPCR and immunohistochemical (IHC) staining. Further, 53 of 80 human ICC specimens (66%) exhibited high LCN2 expression and LCN2 knockdown in SNU308 cells decreased cell growth and migration, suggesting LCN2 be an oncogene in human ICC. As human ICC SNU1079 cells were treated by 1α,25(OH)2D3, LCN2 expression and cell proliferation were attenuated. The downregulation of LCN2 expression was blunted when vitamin D receptor (VDR) was knocked down, implicating that the in vivo Lcn2 downregulation is a direct consequence of vitamin D supplementation Our results support the prevailing concept that vitamin D status is negatively associated with cancer incidence and mortality and suggest LCN2 may be a potential target against ICC. Further studies of application of vitamin D or its analog against ICC are warranted. 出版者： Impact Journals, LLC 出版日期： 2014-05-22 出處： Oncotarget, 2014-05, Vol.5 (11), p.3849-3861 版權： Copyright: © 2014 Chiang et al. 2014 識別號： ISSN: 1949-2553 識別號： EISSN: 1949-2553 識別號： DOI: 10.18632/oncotarget.2000 識別號： PMID: 24939880
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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