Direct detection of orchid viruses using nanorod-based fiber optic particle plasmon resonance immunosensor

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Title:	Direct detection of orchid viruses using nanorod-based fiber optic particle plasmon resonance immunosensor
Authors:	黃貞翰;Lin, Hsing-Ying;Huang, Chen-Han;Lu, Sin-Hong;Kuo, I-Ting;Chau, Lai-Kwan
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	absorption;antibodies;Biological and medical sciences;Biosensors;Biotechnology;color;Cymbidium mosaic virus;detection limit;enzyme-linked immunosorbent assay;Equipment Design;Fiber optic sensor;Fiber Optic Technology - instrumentation;fiber optics;Fundamental and applied biological sciences. Psychology;gold;Gold nanorod;immunosensors;industry;leaves;Limit of Detection;Methods. Procedures. Technologies;nanorods;nanospheres;Nanotubes - chemistry;Odontoglossum ringspot virus;Optical Fibers;Orchid virus detection;Orchidaceae;Orchidaceae - virology;Particle plasmon resonance;Plant Diseases - virology;quality control;quantitative analysis;refractive index;Reproducibility of Results;Surface Plasmon Resonance - instrumentation;Tobamovirus - isolation & purification;Various methods and equipments;viruses
Date:	2014-01-15
Issue Date:	2026-04-23 11:15:43 (UTC+8)
Publisher:	Elsevier Ltd.;Kidlington: Elsevier B.V
Abstract:	摘要： A fiber optic particle plasmon resonance (FOPPR) immunosensor is developed for label-free detection of orchid viruses that use gold nanorods (AuNRs) as the sensing material. The AuNRs are employed to create a near-infrared sensing window to solve the color interference problem of sample matrix for direct sensing of target analyte. This work cannot be achieved using gold nanospheres (AuNSs) because the signal of sample color absorption largely overlaps the signal of molecular recognition events in the visible spectrum, making the signal interpretation much more difficult. The AuNRs are immobilized on the unclad fiber core surface, and functionalized by antibodies which can specifically recognize the corresponding Cymbidium mosaic virus (CymMV) or Odontoglossum ringspot virus (ORSV) for rapid viral infection diagnosis. The refractive index resolution of the AuNR-FOPPR sensor is estimated to be 8×10−6 RIU. The limits of detection (LODs) for CymMV and ORSV in leaf saps are 48 and 42pg/mL, respectively, which are better than the LODs of 1200pg/mL for both viruses obtained by enzyme-linked immunosorbent assay (ELISA). Exploiting the AuNR-FOPPR sensing strategy not only solves the color interference problem encountered by using AuNSs, but provides faster analysis, better reproducibility, and lower detection limit than ELISA. The sensor can distinguish between healthy and infected orchids in 10min, and can further provide the quantitative analysis of infection level. It is potentially applicable to the quality control of orchid cultivation industry, but not limited to this, especially for creating special spectral sensing window for particular samples. •A fiber optic particle plasmon resonance immunosensor based on gold nanorods is developed for label-free detection of orchid viruses.•Employing gold nanorods as the sensing material solves the color interference problem encountered by using gold nanospheres.•Faster analysis, better reproducibility, and lower detection limit than ELISA are provided. 其他題名： Biosens Bioelectron 出版者： Kidlington: Elsevier B.V 出版日期： 2014-01-15 出處： Biosensors & bioelectronics, 2014-01, Vol.51, p.371-378 版權： 2013 Elsevier B.V. 版權： 2014 INIST-CNRS 版權： 2013 Elsevier B.V. All rights reserved. 識別號： ISSN: 0956-5663 識別號： ISSN: 1873-4235 識別號： EISSN: 1873-4235 識別號： DOI: 10.1016/j.bios.2013.08.009 識別號： PMID: 24001513
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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