DNA unmethylome profiling by covalent capture of CpG sites

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Title:	DNA unmethylome profiling by covalent capture of CpG sites
Authors:	王孫崇;Kriukienė, Edita;Labrie, Viviane;Khare, Tarang;Urbanavičiūtė, Giedrė;Lapinaitė, Audronė;Koncevičius, Karolis;Li, Daofeng;Wang, Ting;Pai, Shraddha;Ptak, Carolyn;Gordevičius, Juozas;Wang, Sun-Chong;Petronis, Artūras;Klimašauskas, Saulius
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	631/208/212;631/337/176;Biotin;Biotin - chemistry;Cell Line;CpG Islands;Cytosine - metabolism;Deoxyribonucleic acid;DNA;DNA (Cytosine-5-)-Methyltransferases - genetics;DNA (Cytosine-5-)-Methyltransferases - metabolism;DNA Fingerprinting - methods;DNA Methylation;Epigenesis, Genetic;Genome, Human;Health risks;High-Throughput Nucleotide Sequencing;Humanities and Social Sciences;Humans;Male;multidisciplinary;Oligonucleotide Array Sequence Analysis;Phenotypic variations;Prefrontal Cortex - metabolism;Promoter Regions, Genetic;Science;Science (multidisciplinary);Sequence Analysis, DNA;Spermatozoa - metabolism
Date:	2013-08-02
Issue Date:	2026-04-23 11:15:45 (UTC+8)
Publisher:	Nature Publishing Group;London: Nature Publishing Group UK
Abstract:	摘要： Dynamic patterns of cytosine-5 methylation and successive hydroxylation are part of epigenetic regulation in eukaryotes, including humans, which contributes to normal phenotypic variation and disease risk. Here we present an approach for the mapping of unmodified regions of the genome, which we call the unmethylome. Our technique is based on DNA methyltransferase-directed transfer of activated groups and covalent biotin tagging of unmodified CpG sites followed by affinity enrichment and interrogation on tiling microarrays or next generation sequencing. Control experiments and pilot studies of human genomic DNA from cultured cells and tissues demonstrate that, along with providing a unique cross-section through the chemical landscape of the epigenome, the methyltransferase-directed transfer of activated groups-based approach offers high precision and robustness as compared with existing affinity-based techniques. Chemical modifications of CpG dinucleotides form part of the epigenetic code and various methods for the detection of modified CpG sites exist. Here Kriukiene and colleagues report a complementary method that allows the profiling of unmodified CpG sites within the genome, which they call the 'unmethylome'. 其他題名： Nat Commun 出版者： London: Nature Publishing Group UK 出版日期： 2013 出處： Nature communications, 2013, Vol.4 (1), p.2190, Article 2190 資源來源： Publicly Available Content Database 版權： Springer Nature Limited 2013 版權： Copyright Nature Publishing Group Jul 2013 識別號： ISSN: 2041-1723 識別號： EISSN: 2041-1723 識別號： DOI: 10.1038/ncomms3190 識別號： PMID: 23877302
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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