Enhancing macrophage drug delivery efficiency via co-localization of cells and drug-loaded microcarriers in 3D resonant ultrasound field

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Title:	Enhancing macrophage drug delivery efficiency via co-localization of cells and drug-loaded microcarriers in 3D resonant ultrasound field
Authors:	李宇翔;Lee, Yu-Hsiang;Wu, Zhen-Yu
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Acoustics;Animals;Biomedical engineering;Calcein;Cell Line;Dogs;Drug carriers;Drug delivery;Drug delivery systems;Drug Delivery Systems - methods;Efficiency;Exposure;Flow cytometry;Fluoresceins - chemistry;Genetic engineering;Glycolic acid;Lactic Acid - chemistry;Localization;Macrophages;Macrophages - drug effects;Macrophages - radiation effects;Microspheres;Molecular chains;Polyglycolic Acid - chemistry;Radiation;Sound waves;Ultrasonic imaging;Ultrasonic Waves;Ultrasound
Date:	2015-08-12
Issue Date:	2026-04-23 11:15:55 (UTC+8)
Publisher:	Public Library of Science;United States: Public Library of Science
Abstract:	摘要： In this study, a novel synthetic 3D molecular transfer system which involved the use of model drug calcein-AM-encapsulated poly(lactic-co-glycolic acid) microspheres (CAPMs) and resonant ultrasound field (RUF) with frequency of 1 MHz and output intensity of 0.5 W/cm2 for macrophage drug delivery was explored. We hypothesized that the efficiency of CAPMs-mediated drug delivery aided by RUF can be promoted by increasing the contact opportunities between cells and the micrometer-sized drug carriers due to effects of acoustic radiation forces generated by RUF. Through the fluoromicroscopic and flow cytometric analyses, our results showed that both DH82 macrophages and CAPMs can be quickly brought to acoustic pressure nodes within 20 sec under RUF exposure, and were consequently aggregated throughout the time course. The efficacy of cellular uptake of CAPMs was enhanced with increased RUF exposure time where a 3-fold augmentation (P < 0.05) was obtained after 15 min of RUF exposure. We further demonstrated that the enhanced CAPM delivery efficiency was mainly contributed by the co-localization of cells and CAPMs resulting from the application of the RUF, rather than from sonoporation. In summary, the developed molecular delivery approach provides a feasible means for macrophage drug delivery. 其他題名： PLoS One 出版者： United States: Public Library of Science 出版日期： 2015-08-12 出處： PloS one, 2015-08, Vol.10 (8), p.e0135321 資源來源： Agricultural & Environmental Science Collection 版權： 2015 Lee, Wu. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2015 Lee, Wu 2015 Lee, Wu 識別號： ISSN: 1932-6203 識別號： EISSN: 1932-6203 識別號： DOI: 10.1371/journal.pone.0135321 識別號： PMID: 26267789
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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