Fabrication of keratin/fibroin membranes by electrospinning for vascular tissue engineering

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Title:	Fabrication of keratin/fibroin membranes by electrospinning for vascular tissue engineering
Authors:	陳靖昀;Yen, Ko-Chung;Chen, Ching-Yun;Huang, Jian-Yuan;Kuo, Wei-Ting;Lin, Feng-Huei
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Bypasses;cardiovascular diseases;Cell adhesion;cytotoxicity;death;fibroins;Heart diseases;immunocytochemistry;keratin;Keratins;Medical services;Membranes;microstructure;patients;phenotype;quantitative polymerase chain reaction;scanning electron microscopy;staining;Surgery;tensile strength;tissue engineering;Ultimate tensile strength;wettability
Date:	2015-01-01
Issue Date:	2026-04-23 11:16:03 (UTC+8)
Publisher:	Royal Society of Chemistry;England
Abstract:	摘要： Cardiovascular diseases (CVDs) are some of the leading causes of death and bypass surgery is one of the common treatment options for the critical CVD patients. There is no ideal material available for arterial bypass surgery. Herein, a fibroin and keratin porous membrane was prepared by electro-spinning and proposed for tissue-engineered vascular grafts (TEVGs). The purified fibroin (F) and keratin (K) were mixed in different weight ratios of 9 : 1 (FK91), 8 : 2 (FK82), and 7 : 3 (FK73) to obtain a membrane. The SEM images revealed that the electro-spinned membranes have a fibrous interconnected porous structure. The average diameter of the membrane F, FK91, FK82 and FK73 was 5.74 ± 1.04, 4.20 ± 1.19, 2.94 ± 0.81 and 2.27 ± 0.65 μm, respectively. The ultimate tensile strength (UTS) of F, FK91, FK82 and FK73 was 2.09 ± 0.06, 2.02 ± 0.06, 1.81 ± 0.10 and 1.74 ± 0.12 MPa, respectively. The contact angle of F, FK91, FK82 and FK73 was 72.55 ± 0.55°, 66.39 ± 0.90°, 43.47 ± 0.04° and 33.65 ± 2.83°, respectively. The wettability results were in agreement with those of the cell adhesion to the electro-spinning membranes. The attached HUVECs on the developed membranes showed no cytotoxicity. The immunocytochemistry staining and qPCR analysis showed that the phenotype of the keratin-fibroin membrane was not altered. The results of the ultimate tensile strength, cell adhesion and microstructure revealed that FK82 is similar to native vessels and could be considered as a potential material for TEVGs. Cardiovascular diseases (CVDs) are some of the leading causes of death and bypass surgery is one of the common treatment options for the critical CVD patients. 其他題名： J Mater Chem B 出版者： England 出版日期： 2016-01-14 出處： Journal of materials chemistry. B, Materials for biology and medicine, 2016-01, Vol.4 (2), p.237-244 資源來源： Royal Society of Chemistry 識別號： ISSN: 2050-750X 識別號： ISSN: 2050-7518 識別號： EISSN: 2050-7518 識別號： DOI: 10.1039/c5tb01921d 識別號： PMID: 32263366
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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