Bentham Science Publishers B.V.;United Arab Emirates: Bentham Science Publishers Ltd
摘要:
摘要： Autophagy is an evolutionally conserved process in cells for cleaning abnormal proteins and organelles in a lysosome dependent manner. Growing studies have shown that defects or induced autophagy contributes to many diseases including aging, neurodegeneration, pathogen infection, and cancer. However, the precise involvement of autophagy in health and disease remains controversial because the theories are built on limited assays and chemical modulators, indicating that the role of autophagy in diseases may require further verification. Many food and drug administration (FDA) approved drugs modulate autophagy signaling, suggesting that modulation of autophagy with pharmacological agonists or antagonists provides a potential therapy for autophagy-related diseases. This suggestion raises an attractive issue on drug discovery for exploring chemical modulators of autophagy. High throughput screening (HTS) is becoming a powerful tool for drug discovery that may accelerate screening specific autophagy modulators to clarify the role of autophagy in diseases. Herein, this review lays out current autophagy assays to specifically measure autophagy components such as LC3 (mammalian homologue of yeast Atg8) and Atg4. These assays are feasible or successful for HTS with certain chemical libraries, which might be informative for this intensively growing field as research tools and hopefully developing new drugs for autophagy-related diseases. 其他題名： CCHTS 出版者： United Arab Emirates: Bentham Science Publishers Ltd 出版日期： 2012-11-01 出處： Combinatorial chemistry & high throughput screening, 2012-11, Vol.15 (9), p.721-729 識別號： ISSN: 1386-2073 識別號： ISSN: 1875-5402 識別號： EISSN: 1875-5402 識別號： DOI: 10.2174/138620712803519734 識別號： PMID: 23036098
