Hylan G-F 20 attenuates posttraumatic osteoarthritis progression: Association with upregulated expression of the circardian gene NPAS2

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题名:	Hylan G-F 20 attenuates posttraumatic osteoarthritis progression: Association with upregulated expression of the circardian gene NPAS2
作者:	蘇立仁;Liu, Chih-Chung;Su, Li-Jen;Tsai, Wei-Yuan;Sun, Hsiao-Lun;Lee, Hoong-Chien;Wong, Chih-Shung
贡献者:	生醫理工學院生醫科學與工程學系
关键词:	Animals;anterior cruciate ligament;Anterior Cruciate Ligament Injuries;Arthritis;Basic Helix-Loop-Helix Transcription Factors - drug effects;Basic Helix-Loop-Helix Transcription Factors - genetics;cartilage;Circadian rhythm;Circadian Rhythm - drug effects;Circadian Rhythm - genetics;Disease Progression;Forelimb - injuries;gene expression;Gene Expression - drug effects;gene expression regulation;genes;histopathology;Humans;Hyaluronic acid;Hyaluronic Acid - administration & dosage;Hyaluronic Acid - analogs & derivatives;Hyaluronic Acid - therapeutic use;Hylan;Injections, Intra-Articular;Joints - injuries;Nerve Tissue Proteins - drug effects;Nerve Tissue Proteins - genetics;neurons;NPAS2;osteoarthritis;Osteoarthritis - drug therapy;Osteoarthritis - etiology;Osteoarthritis - pathology;Period Circadian Proteins - biosynthesis;Period Circadian Proteins - genetics;Rats;Rats, Wistar;surgery;synovitis;Up-Regulation - drug effects;Viscosupplements - administration & dosage;Viscosupplements - therapeutic use;Wounds and Injuries - complications
日期:	2015-11-15
上传时间:	2026-04-23 11:16:21 (UTC+8)
出版者:	Elsevier Inc.;Netherlands: Elsevier Inc
摘要:	摘要： The study was to examine the effect of Hylan G-F 20 on the progression of posttraumatic osteoarthritis (PTOA) and the expression of the circadian genes neuronal PAS domain protein 2 (NPAS2) and period 2 (Per2). We used the anterior cruciate ligament transaction and medial menisectomy (ACLT+MMx) model in Wistar rats. The rats were divided into three groups, the sham-operated group, the Hylan G-F 20-treated group, and the saline-treated group. Rats which underwent ACLT + MMx surgery were injected intraarticularly with, respectively, Hylan G-F 20 or saline once a week for 3 consecutive weeks, starting 7days after surgery. The gross morphology and histopathology of the experimental knee joints were evaluated at the end of week 6. Expression of the NPAS2 and Per2 genes was measured by real-time PCR. Hylan G-F 20 suppressed the articular cartilage destruction and synovitis compared to the saline-treated group. Compared to the sham-operated group, the Hylan G-F 20-treated group showed significantly upregulated expression of NPAS2 in cartilage (2.53±0.08-fold higher; p<0.05) and a non-significant increase in Per2 expression (2.35±1.26-fold higher p=0.28), while the saline-treated group showed significant downregulation of NPAS2 expression and a non-significant decrease in Per2 expression. Our data suggested that early intraarticular injection of Hylan G-F 20 attenuates the progression of PTOA and significantly upregulates NPAS2 expression. These findings provide a new direction for studying associations between the use of a pharmacological agent, the degenerative process, and circadian gene expression. 其他題名： Life Sci 出版者： Netherlands: Elsevier Inc 出版日期： 2015-11-15 出處： Life sciences (1973), 2015-11, Vol.141, p.20-24 版權： 2015 Elsevier Inc. 版權： Copyright © 2015 Elsevier Inc. All rights reserved. 識別號： ISSN: 0024-3205 識別號： ISSN: 1879-0631 識別號： EISSN: 1879-0631 識別號： DOI: 10.1016/j.lfs.2015.09.007 識別號： PMID: 26388558
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