Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity

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Title:	Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity
Authors:	李興中;Higuchi, Akon;Kao, Shih-Hsuan;Ling, Qing-Dong;Chen, Yen-Ming;Li, Hsing-Fen;Alarfaj, Abdullah A.;Munusamy, Murugan A.;Murugan, Kadarkarai;Chang, Shih-Chang;Lee, Hsin-Chung;Hsu, Shih-Tien;Kumar, S. Suresh;Umezawa, Akihiro
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	631/532/2117;631/61/54/2295;Amino Acid Sequence;Biomaterials;Cell culture;Cell Culture Techniques - methods;Cell Differentiation;Cell Line;Cell Proliferation;Contamination;Culture Media - chemistry;Elastic Modulus;Embryo cells;Embryo fibroblasts;Embryos;Humanities and Social Sciences;Humans;Hydrogels;Hydrogels - chemistry;Immunohistochemistry;Itaconic acid;Molecular Sequence Data;multidisciplinary;Octamer Transcription Factor-3 - metabolism;Pluripotency;Pluripotent Stem Cells - cytology;Pluripotent Stem Cells - metabolism;Polyvinyl Alcohol - chemistry;Reproducibility of Results;Science;SOXB1 Transcription Factors - metabolism;Stem cell transplantation;Stem cells;Succinates - chemistry;Time Factors;Vitronectin;Vitronectin - chemistry;Xenobiotics - chemistry;Xenografts
Date:	2015-12-14
Issue Date:	2026-04-23 11:16:55 (UTC+8)
Publisher:	Nature Publishing Group;London: Nature Publishing Group UK
Abstract:	摘要： The tentative clinical application of human pluripotent stem cells (hPSCs), such as human embryonic stem cells and human induced pluripotent stem cells, is restricted by the possibility of xenogenic contamination resulting from the use of mouse embryonic fibroblasts (MEFs) as a feeder layer. Therefore, we investigated hPSC cultures on biomaterials with different elasticities that were grafted with different nanosegments. We prepared dishes coated with polyvinylalcohol-co-itaconic acid hydrogels grafted with an oligopeptide derived from vitronectin (KGGPQVTRGDVFTMP) with elasticities ranging from 10.3 to 30.4 kPa storage moduli by controlling the crosslinking time. The hPSCs cultured on the stiffest substrates (30.4 kPa) tended to differentiate after five days of culture, whereas the hPSCs cultured on the optimal elastic substrates (25 kPa) maintained their pluripotency for over 20 passages under xeno-free conditions. These results indicate that cell culture matrices with optimal elasticity can maintain the pluripotency of hPSCs in culture. 其他題名： Sci Rep 出版者： London: Nature Publishing Group UK 出版日期： 2015-12-14 出處： Scientific reports, 2015-12, Vol.5 (1), p.18136-18136, Article 18136 資源來源： Publicly Available Content Database (Proquest) 版權： The Author(s) 2015 版權： Copyright Nature Publishing Group Dec 2015 版權： Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited 識別號： ISSN: 2045-2322 識別號： EISSN: 2045-2322 識別號： DOI: 10.1038/srep18136 識別號： PMID: 26656754
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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