miR-524-5p suppresses the growth of oncogenic BRAF melanoma by targeting BRAF and ERK2

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題名:	miR-524-5p suppresses the growth of oncogenic BRAF melanoma by targeting BRAF and ERK2
作者:	馬念涵;Liu, Szu-Mam;Lu, Jean;Lee, Hoong-Chien;Chung, Feng-Hsiang;Ma, Nianhan
貢獻者:	生醫理工學院生醫科學與工程學系
關鍵詞:	3' Untranslated Regions - genetics;Animals;Apoptosis - genetics;Cell Cycle - genetics;Cell Line, Tumor;Cell Movement - genetics;Cell Proliferation - genetics;Gene Expression Regulation, Neoplastic;HEK293 Cells;Humans;Male;MAP Kinase Signaling System - genetics;Melanoma - genetics;Melanoma - pathology;Mice;Mice, Inbred NOD;Mice, SCID;MicroRNAs - biosynthesis;MicroRNAs - genetics;Mitogen-Activated Protein Kinase 1 - biosynthesis;Mitogen-Activated Protein Kinase 1 - genetics;Mitogen-Activated Protein Kinase 1 - metabolism;Neoplasm Transplantation;Proto-Oncogene Proteins B-raf - biosynthesis;Proto-Oncogene Proteins B-raf - genetics;Proto-Oncogene Proteins B-raf - metabolism;Research Paper;Transplantation, Heterologous
日期:	2014-01-01
上傳時間:	2026-04-23 11:17:19 (UTC+8)
出版者:	United States: Impact Journals, LLC
摘要:	摘要： It has been well documented that miRNAs can modulate the effectiveness of cancer-associated signaling pathways. Mitogen-activated protein kinase (MAPK/ERK) signaling plays an essential role in the progression of many cancers, including melanoma and colon cancers. However, no single miRNA is reported to directly target multiple components of the MAPK/ERK pathway. We performed a miRNA PCR array screening with various MAPK/ERK signaling activities. The miRNA array data revealed that the expression of miR-524-5p was decreased in cells with an active MAPK/ERK pathway and confirmed that the expression of miR-524-5p is inversely associated with the activity of the MAPK/ERK pathway. We demonstrated that miR-524-5p directly binds to the 3'-untranslated regions of both BRAFandERK2 and suppresses the expression of these proteins. Because BRAF and ERK2 are the main components of MAPK signaling, the overexpression of miR-524-5p effectively inhibits MAPK/ERK signaling, tumor proliferation, and melanoma cell migration. Moreover, tumors overexpressing miR-524-5p were significantly smaller than those of the negative control mice. Our findings provide new insight into the role of miR-524-5p as an important miRNA that negatively regulates the MAPK/ERK signaling pathway, suggesting that miR-524-5p could be a potent therapeutic candidate for melanoma treatment. 其他題名： Oncotarget 出版者： United States: Impact Journals, LLC 出版日期： 2014-10-15 出處： Oncotarget, 2014-10, Vol.5 (19), p.9444-9459 版權： Copyright: © 2014 Liu et al. 2014 識別號： ISSN: 1949-2553 識別號： EISSN: 1949-2553 識別號： DOI: 10.18632/oncotarget.2452 識別號： PMID: 25275294
顯示於類別:	[生醫科學與工程學系] 期刊論文

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