Nasal commensal Staphylococcus epidermidis counteracts influenza virus

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題名:	Nasal commensal Staphylococcus epidermidis counteracts influenza virus
作者:	黃俊銘;Chen, Hui-Wen;Liu, Pei-Feng;Liu, Yu-Tsueng;Kuo, Sherwin;Zhang, Xing-Quan;Schooley, Robert T.;Rohde, Holger;Gallo, Richard L.;Huang, Chun-Ming
貢獻者:	生醫理工學院生醫科學與工程學系
關鍵詞:	631/326/1320;692/699/1785;82;82/83;Bacteria;Chickens;Humanities and Social Sciences;Liquid chromatography;Mass spectrometry;multidisciplinary;Pathogens;Prevention;Science;Staphylococcus infections;Survival;Vaccines;Viral infections;Viruses
日期:	2016-06-16
上傳時間:	2026-04-23 11:17:40 (UTC+8)
出版者:	Nature Publishing Group;London: Springer Science and Business Media LLC
摘要:	摘要： AbstractSeveral microbes, including Staphylococcus epidermidis (S. epidermidis), a Gram-positive bacterium, live inside the human nasal cavity as commensals. The role of these nasal commensals in host innate immunity is largely unknown, although bacterial interference in the nasal microbiome may promote ecological competition between commensal bacteria and pathogenic species. We demonstrate here that S. epidermidis culture supernatants significantly suppressed the infectivity of various influenza viruses. Using high-performance liquid chromatography together with mass spectrometry, we identified a giant extracellular matrix-binding protein (Embp) as the major component involved in the anti-influenza effect of S. epidermidis. This anti-influenza activity was abrogated when Embp was mutated, confirming that Embp is essential for S. epidermidis activity against viral infection. We also showed that both S. epidermidis bacterial particles and Embp can directly bind to influenza virus. Furthermore, the injection of a recombinant Embp fragment containing a fibronectin-binding domain into embryonated eggs increased the survival rate of virus-infected chicken embryos. For an in vivo challenge study, prior Embp intranasal inoculation in chickens suppressed the viral titres and induced the expression of antiviral cytokines in the nasal tissues. These results suggest that S. epidermidis in the nasal cavity may serve as a defence mechanism against influenza virus infection. 其他題名： Sci Rep 出版者： London: Springer Science and Business Media LLC 出版日期： 2016-06-16 出處： Scientific Reports, 2016-06, Vol.6 (1), p.27870--27870, Article 27870 資源來源： Springer Nature Link 版權： The Author(s) 2016 版權： Copyright Nature Publishing Group Jun 2016 版權： Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited 識別號： ISSN: 2045-2322 識別號： EISSN: 2045-2322 識別號： DOI: 10.1038/srep27870 識別號： PMID: 27306590
顯示於類別:	[生醫科學與工程學系] 期刊論文

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