On-line SERS detection of single bacterium Using Novel SERS Nanoprobes and a microfl uidic Dielectrophoresis device

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Title:	On-line SERS detection of single bacterium Using Novel SERS Nanoprobes and a microfl uidic Dielectrophoresis device
Authors:	黃貞翰;Lin, Hsing-Ying;Huang, Chen-Han;Hsieh, Wen-Hsin;Liu, Ling-Hsuan;Lin, Yuan-Chuen;Chu, Chia-Chun;Wang, Shi-Ting;Kuo, I-Ting;Chau, Lai-Kwan;Yang, Chiou-Ying
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Assaying;Bacteria;biosensors;Dielectrophoresis;Electrophoresis - instrumentation;Enzyme-Linked Immunosorbent Assay;Immunoassay;Metal Nanoparticles;Microfluidics - instrumentation;Microscopy, Electron, Transmission;Molecular Probes;Multiplexing;Nanostructure;Nanotechnology;Neisseria lactamica;On-line systems;Raman scattering;raman spectroscopy;Salmonella enterica;Salmonella enterica - isolation & purification;SERS nanoprobes;Spectrum analysis;Spectrum Analysis, Raman - methods;Strategy
Date:	2014-11-26
Issue Date:	2026-04-23 11:18:22 (UTC+8)
Publisher:	Wiley-VCH Verlag;Germany: Blackwell Publishing Ltd
Abstract:	摘要： The integration of novel surface‐enhanced Raman scattering (SERS) nanoprobes and a microfluidic dielectrophoresis (DEP) device is developed for rapid on‐line SERS detection of Salmonella enterica serotype Choleraesuis and Neisseria lactamica. The SERS nanoprobes are prepared by immobilization of specific antibody onto the surface of nanoaggregate‐embedded beads (NAEBs), which are silica‐coated, dye‐induced aggregates of a small number of gold nanoparticles (AuNPs). Each NAEB gives highly enhanced Raman signals owing to the presence of well‐defined plasmonic hot spots at junctions between AuNPs. Herein, the on‐line SERS detection and accurate identification of suspended bacteria with a detection capability down to a single bacterium has been realized by the NAEB−DEP−Raman spectroscopy biosensing strategy. The practical detection limit with a measurement time of 10 min is estimated to be 70 CFU mL−1. In comparison with whole‐cell enzyme‐linked immunosorbent assay (ELISA), the SERS‐nanoprobe‐based biosensing method provides advantages of higher sensitivity and requiring lower amount of antibody in the assay (100‐fold less). The total assay time including sample pretreatment is less than 2 h. Hence, this sensing strategy is promising for faster and effective on‐line multiplex detection of single pathogenic bacterium by using different bioconjugated SERS nanoprobes. 其他題名： Small 出版者： Germany: Blackwell Publishing Ltd 出版日期： 2014-11 出處： Small, 2014-11, Vol.10 (22), p.4700-4710 版權： 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim 版權： 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 版權： Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim 識別號： ISSN: 1613-6810 識別號： ISSN: 1613-6829 識別號： EISSN: 1613-6829 識別號： DOI: 10.1002/smll.201401526 識別號： PMID: 25115777
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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