Radix Scrophulariae extracts (harpagoside) suppresses hypoxia-induced microglial activation and neurotoxicity

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Title:	Radix Scrophulariae extracts (harpagoside) suppresses hypoxia-induced microglial activation and neurotoxicity
Authors:	陳靖昀;Sheu, Shiow-Yunn;Hong, Yi-Wen;Sun, Jui-Sheng;Liu, Man-Hai;Chen, Ching-Yun;Ke, Cherng-Jyh
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Animals;Chiropractic Medicine;complement;Complementary & Alternative Medicine;cytosol;Cytotoxicity;Gene expression;Gene Expression - drug effects;Genes;Glycosides - pharmacology;Hypoxia;Hypoxia - metabolism;Inflammation;Inflammation - metabolism;interleukin-6;Internal Medicine;Medicine;Medicine & Public Health;Mice;Mice, Inbred ICR;Microglia - drug effects;neuroglia;neurons;Neurotoxicity;Physiological aspects;Plant Extracts - pharmacology;Protective Agents - pharmacology;protein phosphorylation;protein transport;Pyrans - pharmacology;Research Article;Scrophularia - chemistry;toxicity testing;Translocation
Date:	2015-09-14
Issue Date:	2026-04-23 11:19:55 (UTC+8)
Publisher:	BioMed Central Ltd.;London: BioMed Central
Abstract:	摘要： Background Hypoxia could lead to microglia activation and inflammatory mediators’ overproduction. These inflammatory molecules could amplify the neuroinflammatory process and exacerbate neuronal injury. The aim of this study is to find out whether harpagoside could reduce hypoxia-induced microglia activation. Methods In this study, primary microglia cells harvested from neonatal ICR mice were activated by exposure to hypoxia (1 % O 2 for 3 h). Harpagoside had been shown to be no cytotoxicity on microglia cells by MTT assay. The scavenger effect of harpagoside on hypoxia-enhanced microglial cells proliferation, associated inflammatory genes expression (COX-II, IL-1β and IL-6 genes) and NO synthesis were also examined. Results Hypoxia enhances active proliferation of microglial cells, while harpagoside can scavenge this effect. We find that harpagoside could scavenge hypoxia-enhanced inflammatory genes expression (COX-2, IL-1β and IL-6 genes) and NO synthesis of microglial cells. Under 3 h’ hypoxic stimulation, the nuclear contents of p65 and hypoxia inducible factor-1α (HIF-1α) significantly increase, while the cytosol IκB-α content decreases; these effects can be reversed by 1 h’s pre-incubation of 10 −8 M harpagoside. Harpagoside could decrease IκB-α protein phosphorylation and inhibit p65 protein translocation from the cytosol to the nucleus, thus suppress NF-κB activation and reduce the HIF-1α generation. Conclusion These results suggested that the anti-inflammatory mechanism of harpagoside might be associated with the NF-κB signaling pathway. Harpagoside protect against hypoxia-induced toxicity on microglial cells through HIF-α pathway. 其他題名： BMC Complement Altern Med 出版者： London: BioMed Central 出版日期： 2015-09-14 出處： BMC complementary and alternative medicine, 2015-09, Vol.15 (1), p.324-324, Article 324 資源來源： PubMed Central Open Access 版權： Sheu et al. 2015 版權： COPYRIGHT 2015 BioMed Central Ltd. 版權： Copyright BioMed Central 2015 識別號： ISSN: 1472-6882 識別號： EISSN: 1472-6882 識別號： DOI: 10.1186/s12906-015-0842-x 識別號： PMID: 26370415
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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