資料載入中.....
|
請使用永久網址來引用或連結此文件:
https://ir.lib.ncu.edu.tw/handle/987654321/102936
|
| 題名: | ROS-induced toxicity: exposure of 3T3, RAW264.7, and MCF7 cells to superparamagnetic iron oxide nanoparticles results in cell death by mitochondria-dependent apoptosis |
| 作者: | 陳靖昀;Hsieh, Hui-Chen;Chen, Chung-Ming;Hsieh, Wen-Yuan;Chen, Ching-Yun;Liu, Chia-Ching;Lin, Feng-Huei |
| 貢獻者: | 生醫理工學院生醫科學與工程學系 |
| 關鍵詞: | Apoptosis;Cancer;Cell death;Cellular;Characterization and Evaluation of Materials;Chemistry and Materials Science;Correlation analysis;Cytotoxicity;Inorganic Chemistry;Iron oxides;Lasers;Materials Science;Mitochondria;Mortality;Nanoparticles;Nanotechnology;Optical Devices;Optics;Oxidative stress;Pathways;Photonics;Physical Chemistry;Research Paper;Stresses;Transmission electron microscopy |
| 日期: | 2015-02-01 |
| 上傳時間: | 2026-04-23 11:20:28 (UTC+8) |
| 出版者: | Springer Netherlands;Dordrecht: Springer Netherlands |
| 摘要: | 摘要： Superparamagnetic nanoparticles (Fe 3 O 4 , SPIO) have been used as magnetic resonance imaging enhancers for years. However, bio-safety issues concerning nanoparticles remain largely unexplored. Of particular concern is the possible cellular impact of nanoparticles during SPIO uptake and subsequent oxidative stress. SPIO causes cell death by apoptosis via a little understood mitochondrial pathway. To more closely examine this process, three kinds of cells—3T3, RAW264.7, and MCF7—were treated with SPIO coated with polyethylene glycol (SPIO-PEG) and monitored by transmission electron microscopy (TEM), using cytotoxicity evaluation, mitochondrial activity, reactive oxygen species (ROS) generation, and Annexin V assay. TEM revealed that SPIO-PEG nanoparticles surrounded the cellular endosome membrane, creating a bulge in the endosome. Compared to 3T3 cells, greater numbers of SPIO-PEG nanoparticles infiltrated the mitochondria of RAW264.7 and MCF7 cells. SPIO-PEG residency is associated with boosted ROS, with elevated levels of mitochondrial activity, and advancement of cell apoptosis. Furthermore, correlation analysis showed that a polynomial model demonstrates a better fit than a linear model in MCF7, implying that cytotoxicity may have alternative impacts on cell death at different concentrations. Thus, we believe that MCF7 cell death results from the apoptosis pathway triggered by mitochondria, and we find lower cytotoxicity in 3T3. We propose that optimal levels of SPIO-PEG nanoparticles lead to increased levels of ROS and a resulting oxidative stress environment which will kill only cancer cells while sparing normal cells. This finding has great potential for use in cancer therapies in the future.
其他題名： J Nanopart Res
出版者： Dordrecht: Springer Netherlands
出版日期： 2015-02
出處： Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology, 2015-02, Vol.17 (2), p.1-14, Article 71
版權： Springer Science+Business Media Dordrecht 2015
識別號： ISSN: 1388-0764
識別號： EISSN: 1572-896X
識別號： DOI: 10.1007/s11051-015-2886-8 |
| 顯示於類別: | [生醫科學與工程學系] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| index.html | | 0Kb | HTML | 18 | 檢視/開啟 |
|
在NCUIR中所有的資料項目都受到原著作權保護.
|
