Secretome profiling of primary cells reveals that THBS2 is a salivary biomarker of oral cavity squamous cell carcinoma

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題名:	Secretome profiling of primary cells reveals that THBS2 is a salivary biomarker of oral cavity squamous cell carcinoma
作者:	劉淑貞;Hsu, Chia-Wei;Yu, Jau-Song;Peng, Pei-Hua;Liu, Shu-Chen;Chang, Yu-Sun;Chang, Kai-Ping;Wu, Chih-Ching
貢獻者:	生醫理工學院生醫科學與工程學系
關鍵詞:	biomarkers;Biomarkers, Tumor - metabolism;Carcinoma, Squamous Cell - diagnosis;Carcinoma, Squamous Cell - metabolism;cell culture;Chromatography, Liquid;Computational Biology;data collection;death;Electrophoresis, Polyacrylamide Gel;epithelium;gene expression;Humans;Immunoassay;Immunohistochemistry;Male;messenger RNA;Middle Aged;mortality;mouth;mouth neoplasms;Mouth Neoplasms - diagnosis;Mouth Neoplasms - metabolism;patients;prognosis;proteins;proteome;Proteomics - methods;Saliva - secretion;squamous cell carcinoma;Statistics, Nonparametric;Taiwan;Tandem Mass Spectrometry;Thrombospondins - secretion;transcriptome;Tumor Cells, Cultured
日期:	2014-01-01
上傳時間:	2026-04-23 11:20:34 (UTC+8)
出版者:	American Chemical Society;United States: American Chemical Society
摘要:	摘要： Oral cavity squamous cell carcinoma (OSCC), which is a leading cause of cancer-related death worldwide, is frequently associated with poor prognosis and mortality. The discovery of body fluid-accessible biomarkers may help improve the detection of OSCC. In the present work, we established primary cell cultures derived from OSCC and adjacent noncancerous epithelium and performed comparative profiling of their secretomes. Using spectral counting-based label-free quantification, we found that 64 proteins were significantly higher in primary OSCC cells compared with primary adjacent noncancerous cells. We then retrieved the mRNA expression levels of these 64 proteins in oral cavity tumor and noncancerous tissues from public domain array-based transcriptome data sets and used this information to prioritize the biomarker candidates. We identified 19 candidates; among them, the protein levels of THBS2, UFD1L, and DNAJB11 were found to be elevated in OSCC tissues compared with adjacent noncancerous epithelia. Importantly, higher levels of THBS2 in OSCC tissues were associated with a higher overall pathological stage, positive perineural invasion, and a poorer prognosis. Moreover, the salivary levels of THBS2 in OSCC patients were elevated compared to those of noncancer controls. Our results collectively indicate that analysis of the primary cell secretome is a feasible strategy for biomarker identification, and that THBS2 is a potentially useful salivary marker for the detection of OSCC. 其他題名： J. Proteome Res 出版者： United States: American Chemical Society 出版日期： 2014-11-07 出處： Journal of proteome research, 2014-11, Vol.13 (11), p.4796-4807 資源來源： American Chemical Society Journals 版權： Copyright © 2014 American Chemical Society 識別號： ISSN: 1535-3893 識別號： ISSN: 1535-3907 識別號： EISSN: 1535-3907 識別號： DOI: 10.1021/pr500038k 識別號： PMID: 24708169
顯示於類別:	[生醫科學與工程學系] 期刊論文

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