The Motor Protein KIF14 Inhibits Tumor Growth and Cancer Metastasis in Lung Adenocarcinoma

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Title:	The Motor Protein KIF14 Inhibits Tumor Growth and Cancer Metastasis in Lung Adenocarcinoma
Authors:	許藝瓊;Hung, Pei-Fang;Hong, Tse-Ming;Hsu, Yi-Chiung;Chen, Hsuan-Yu;Chang, Yih-Leong;Wu, Chen-Tu;Chang, Gee-Chen;Jou, Yuh-Shan;Pan, Szu-Hua;Yang, Pan-Chyr
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Adenocarcinoma;Adenocarcinoma - genetics;Adenocarcinoma - metabolism;Adenocarcinoma - mortality;Adenocarcinoma - pathology;Adenocarcinoma of Lung;Adhesion;Aged;Anaphase;Animals;Biology;Brain cancer;Bronchi - cytology;Bronchi - metabolism;Cadherins;Cadherins - genetics;Cadherins - metabolism;Cancer;Cancer metastasis;Cancer research;Cancer treatment;CD146 Antigen - genetics;CD146 Antigen - metabolism;Cell Adhesion;Cell adhesion molecules;Cell cycle;Cell migration;Cell Movement;Chromosome 1;Chromosomes;Collagen (type IV);Collagen Type IV - metabolism;Comparative analysis;Comparative Genomic Hybridization;Cytogenetics;Development and progression;Epithelial cells;Epithelial Cells - cytology;Epithelial Cells - metabolism;Extracellular matrix;Female;Gene expression;Gene Expression Regulation, Neoplastic;Genomics;Growth;Heterozygosity;Humans;Hybridization;Internal medicine;Invasiveness;Kinases;Kinesin;Kinesin - antagonists & inhibitors;Kinesin - genetics;Kinesin - metabolism;Laminin;Laminin - metabolism;Liver cancer;Loss of heterozygosity;Lung cancer;Lung Neoplasms - genetics;Lung Neoplasms - metabolism;Lung Neoplasms - mortality;Lung Neoplasms - pathology;Male;Medicine;Melanoma;Metaphase;Metastases;Metastasis;Mice;Mice, SCID;Middle Aged;mRNA;Neoplasm Metastasis;Oncogene Proteins - antagonists & inhibitors;Oncogene Proteins - genetics;Oncogene Proteins - metabolism;Pathology;Prognosis;Protein Binding;Protein expression;Proteins;Proteomics;RNA;RNA, Small Interfering - genetics;RNA, Small Interfering - metabolism;Survival Analysis;Tumorigenesis;Tumors;Xenograft Model Antitumor Assays;Xenografts
Date:	2013-04-23
Issue Date:	2026-04-23 11:22:55 (UTC+8)
Publisher:	Public Library of Science;United States: Public Library of Science
Abstract:	摘要： The motor protein kinesin superfamily proteins (KIFs) are involved in cancer progression. The depletion of one of the KIFs, KIF14, might delay the metaphase-to-anaphase transition, resulting in a binucleated status, which enhances tumor progression; however, the exact correlation between KIF14 and cancer progression remains ambiguous. In this study, using loss of heterozygosity and array comparative genomic hybridization analyses, we observed a 30% loss in the regions surrounding KIF14 on chromosome 1q in lung adenocarcinomas. In addition, the protein expression levels of KIF14 in 122 lung adenocarcinomas also indicated that approximately 30% of adenocarcinomas showed KIF14 down-regulation compared with the expression in the bronchial epithelial cells of adjacent normal counterparts. In addition, the reduced expression of KIF14 mRNA or proteins was correlated with poor overall survival (P = 0.0158 and <0.0001, respectively), and the protein levels were also inversely correlated with metastasis (P<0.0001). The overexpression of KIF14 in lung adenocarcinoma cells inhibited anchorage-independent growth in vitro and xenograft tumor growth in vivo. The overexpression and silencing of KIF14 also inhibited or enhanced cancer cell migration, invasion and adhesion to the extracellular matrix proteins laminin and collagen IV. Furthermore, we detected the adhesion molecules cadherin 11 (CDH11) and melanoma cell adhesion molecule (MCAM) as cargo on KIF14. The overexpression and silencing of KIF14 enhanced or reduced the recruitment of CDH11 in the membrane fraction, suggesting that KIF14 might act through recruiting adhesion molecules to the cell membrane and modulating cell adhesive, migratory and invasive properties. Thus, KIF14 might inhibit tumor growth and cancer metastasis in lung adenocarcinomas. 其他題名： PLoS One 出版者： United States: Public Library of Science 出版日期： 2013-04-23 出處： PloS one, 2013-04, Vol.8 (4), p.e61664 資源來源： Agricultural & Environmental Science Collection 版權： COPYRIGHT 2013 Public Library of Science 版權： 2013 Hung et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2013 Hung et al 2013 Hung et al 識別號： ISSN: 1932-6203 識別號： EISSN: 1932-6203 識別號： DOI: 10.1371/journal.pone.0061664 識別號： PMID: 23626713
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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