The Response of Human Skin Commensal Bacteria as a Reflection of UV Radiation: UV-B Decreases Porphyrin Production

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Title:	The Response of Human Skin Commensal Bacteria as a Reflection of UV Radiation: UV-B Decreases Porphyrin Production
Authors:	黃俊銘;Wang, Yanhan;Zhu, Wenhong;Shu, Muya;Jiang, Yong;Gallo, Richard L.;Liu, Yu-Tsueng;Huang, Chun-Ming
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Acne;Amino Acid Sequence;Amino acids;Animal tissues;Animals;Bacteria;Biology;Biomarkers;Broadway theater;Cancer;Cancer treatment;Compounds;Cyclobutane;Cyclobutane pyrimidine dimers;Dermatology;Development and progression;Dimers;Dose-Response Relationship, Radiation;Exposure;Gamma rays;Global radiation;Health aspects;Health risks;Human behavior;Human tissues;Humans;Illnesses;Immune status;Mass spectrometry;Medicine;Mice;Molecular Sequence Data;Neoplasms - prevention & control;Nuclear accidents;Nuclear accidents & safety;Oxidation;Peptides;Peptides - chemistry;Physics;Porphyrins;Porphyrins - biosynthesis;Production management;Propionibacterium;Propionibacterium acnes - metabolism;Quadrupoles;Radiation;Release factor 2;Risk factors;Skin;Skin - microbiology;Skin - radiation effects;Skin cancer;Spectrometry;Spectrometry, Fluorescence - methods;Staphylococcus epidermidis;Terrorism;Tumor necrosis factor-TNF;Ultraviolet radiation;Ultraviolet Rays;γ Radiation
Date:	2012-10-25
Issue Date:	2026-04-23 11:22:59 (UTC+8)
Publisher:	Public Library of Science;United States: Public Library of Science
Abstract:	摘要： Recent global radiation fears reflect the urgent need for a new modality that can simply determine if people are in a radiation risk of developing cancer and other illnesses. Ultraviolet (UV) radiation has been thought to be the major risk factor for most skin cancers. Although various biomarkers derived from the responses of human cells have been revealed, detection of these biomarkers is cumbersome, probably requires taking live human tissues, and varies significantly depending on human immune status. Here we hypothesize that the reaction of Propionibacterium acnes (P. acnes), a human resident skin commensal, to UV radiation can serve as early surrogate markers for radiation risk because the bacteria are immediately responsive to radiation. In addition, the bacteria can be readily accessible and exposed to the same field of radiation as human body. To test our hypothesis, P. acnes was exposed to UV-B radiation. The production of porphyrins in P. acnes was significantly reduced with increasing doses of UV-B. The porphyrin reduction can be detected in both P. acnes and human skin bacterial isolates. Exposure of UV-B to P. acnes- inoculated mice led to a significant decrease in porphyrin production in a single colony of P. acnes and simultaneously induced the formation of cyclobutane pyrimidine dimers (CPD) in the epidermal layers of mouse skin. Mass spectrometric analysis via a linear trap quadrupole (LTQ)-Orbitrap XL showed that five peptides including an internal peptide (THLPTGIVVSCQNER) of a peptide chain release factor 2 (RF2) were oxidized by UV-B. Seven peptides including three internal peptides of 60 kDa chaperonin 1 were de-oxidized by UV-B. When compared to UV-B, gamma radiation also decreased the porphyrin production of P. acnes in a dose-dependent manner, but induced a different signature of protein oxidation/de-oxidation. We highlight that uncovering response of skin microbiome to radiation will facilitate the development of pre-symptomatic diagnosis of radiation risk in a battlefield exposure, nuclear accidents, terrorist attacks, or cancer imaging/therapy. 其他題名： PLoS One 出版者： United States: Public Library of Science 出版日期： 2012-10-25 出處： PloS one, 2012-10, Vol.7 (10), p.e47798 資源來源： Agricultural & Environmental Science Collection 版權： COPYRIGHT 2012 Public Library of Science 版權： 2012 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 版權： 2012 Wang et al 2012 Wang et al 識別號： ISSN: 1932-6203 識別號： EISSN: 1932-6203 識別號： DOI: 10.1371/journal.pone.0047798 識別號： PMID: 23133525
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