Tubular waveguide evanescent field absorption biosensor based on particle plasmon resonance for multiplex label-free detection

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Title:	Tubular waveguide evanescent field absorption biosensor based on particle plasmon resonance for multiplex label-free detection
Authors:	黃貞翰;Lin, Hsing-Ying;Huang, Chen-Han;Chen, Shin-Huei;Liu, Yu-Chia;Chang, Wei-Zhe;Chau, Lai-Kwan
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Absorption;antibodies;Biological and medical sciences;Biological Assay - instrumentation;Biosensing Techniques - instrumentation;Biosensor;Biosensors;Biotechnology;coatings;detection limit;Equipment Design;Equipment Failure Analysis;Fundamental and applied biological sciences. Psychology;High-throughput biochemical detection;Immunoassay - instrumentation;Light;Localized surface plasmon resonance;Methods. Procedures. Technologies;monitoring;Nanoparticle;Optical waveguide;refractive index;Refractometry - instrumentation;Reproducibility of Results;screening;Sensitivity and Specificity;Staining and Labeling;streptavidin;Surface Plasmon Resonance - instrumentation;urine;Various methods and equipments
Date:	2013-03-15
Issue Date:	2026-04-23 11:23:12 (UTC+8)
Publisher:	Elsevier Ltd.;Kidlington: Elsevier B.V
Abstract:	摘要： A novel tubular waveguide particle plasmon resonance (TW-PPR) sensor is demonstrated for label-free biochemical detection. The sensor itself is a microchamber of a defined sample volume, a mechanical support for sensor coating, a waveguide to provide evanescent wave interrogation, and it can be easily extended to a multi-channel format. The sensor resolution is estimated to be 2.6×10−6RIU in measuring solutions of various refractive indices. The sensing system can perform multiple measurements simultaneously and its limit of detection for anti-DNP antibody and streptavidin is 1.2×10−10g/ml (0.55pM) and 2.3×10−10g/ml (3.5pM), respectively. Accurate determination of these analytes with known concentration spiked in artificial urine were examined and the bias is less than ±7%, supporting the utility of the device for analyte screening in more complex media. The TW-PPR sensor can be inexpensively fabricated and has a special niche for monitoring biomolecular interactions in real-time, hence it is ideally suitable for disposable uses, especially promising for convenient high-throughput biochemical sensing applications. ► A tubular waveguide particle plasmon resonance sensor is used for label-free biochemical sensing. ► It relies on an unsophisticated light intensity interrogation scheme. ► A multi-channel sensing system is developed for higher-throughput biochemical detection. 其他題名： Biosens Bioelectron 出版者： Kidlington: Elsevier B.V 出版日期： 2013-03-15 出處： Biosensors & bioelectronics, 2013-03, Vol.41, p.268-274 版權： 2012 Elsevier B.V. 版權： 2014 INIST-CNRS 版權： Copyright © 2012 Elsevier B.V. All rights reserved. 識別號： ISSN: 0956-5663 識別號： ISSN: 1873-4235 識別號： EISSN: 1873-4235 識別號： DOI: 10.1016/j.bios.2012.08.041 識別號： PMID: 22964381
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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