Using polyethylene glycol as nonionic osmoticum to promote growth and lipid production of marine microalgae Nannochloropsis oculata

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Title:	Using polyethylene glycol as nonionic osmoticum to promote growth and lipid production of marine microalgae Nannochloropsis oculata
Authors:	李宇翔;Lee, Yu-Hsiang;Yeh, Yu-Ling
Contributors:	生醫理工學院生醫科學與工程學系
Keywords:	Abiotic stress;Adaptability;Algae;Biomass;Biotechnology;Chemistry;Chemistry and Materials Science;Cultivation;economic feasibility;eicosapentaenoic acid;Environmental Engineering/Biotechnology;Food Science;growth promotion;Industrial and Production Engineering;Industrial Chemistry/Chemical Engineering;lipid content;Lipids;Lipids - biosynthesis;Microalgae;molecular weight;Nannochloropsis;Nannochloropsis oculata;Original Paper;Osmotic Pressure - drug effects;osmotic stress;oxygen;Polyethylene glycol;Polyethylene Glycols - pharmacology;Solvents - pharmacology;Stramenopiles - growth & development
Date:	2014-08-01
Issue Date:	2026-04-23 11:23:21 (UTC+8)
Publisher:	Springer Verlag;Berlin/Heidelberg: Springer Berlin Heidelberg
Abstract:	摘要： To promote the economic feasibility of Nannochloropsis oculata , efficacy of using polyethylene glycol (PEG) to increase microalgal growth and lipid accumulation was investigated. We first examined the effects of PEG concentrations on microalgal growth using 0–5 % (w/v) PEG-6000, and followed by exploring the effects of PEG molecular weights (400, 600, 2,000, 4,000, 6,000, and 20,000) on microalgal growth, size, as well as on yields of biomass, total lipids, and eicosapentaenoic acid. In addition, the capacity of PEG to reduce the effect of oxygen inhibition on microalgal growth was also investigated to evaluate its adaptability for use in large-scale and closed setting. Our results showed that PEG-induced osmotic stress ( Π ) in the range of 2.465–2.472 MPa can raise microalgal growth. The PEG with higher molecular weight exhibited greater efficacy of growth promotion but less lipid content under equal concentration. In this study, 0.5 % (w/v) PEG-20000 ( Π = 2.466 MPa) remarkably enhanced microalgal growth without interference of intracellular lipid productivity and cellular size, yielding >50 % (w/w) increases in biomass, total lipid, and eicosapentaenoic acid amounts after 7 days that provided the optimal condition for microalgal cultivation. These positive effects possibly resulted from the moderate enhancement of osmotic stress in the medium and stronger chaotrope-like behavior from higher molecular weight PEG. With further verification that 0.5 % (w/v) PEG-20000 enabled to reduce the effect of oxygen inhibition on microalgal growth, the PEG-20000-mediated cultivation offers a feasible means for mass culture of N. oculata in closed setting. 其他題名： Bioprocess Biosyst Eng 出版者： Berlin/Heidelberg: Springer Berlin Heidelberg 出版日期： 2014-08-01 出處： Bioprocess and biosystems engineering, 2014-08, Vol.37 (8), p.1669-1677 版權： Springer-Verlag Berlin Heidelberg 2014 識別號： ISSN: 1615-7591 識別號： ISSN: 1615-7605 識別號： EISSN: 1615-7605 識別號： DOI: 10.1007/s00449-014-1139-z 識別號： PMID: 24522612
Appears in Collections:	[Department of Biomedical Sciences and Engineering ] journal & Dissertation

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