Approach to Deliver Two Antioxidant Enzymes with Mesoporous Silica Nanoparticles into Cells

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/103106

Title:	Approach to Deliver Two Antioxidant Enzymes with Mesoporous Silica Nanoparticles into Cells
Authors:	簡汎清;Lin, Yu-Hsuan;Chen, Yi-Ping;Liu, Tsang-Pai;Chien, Fan-Ching;Chou, Chih-Ming;Chen, Chien-Tsu;Mou, Chung-Yuan
Contributors:	理學院光電科學與工程學系
Keywords:	Antioxidants - administration & dosage;Antioxidants - chemistry;cell cycle checkpoints;cell membranes;cytoskeleton;fluorescent dyes;glutathione peroxidase;Glutathione Peroxidase - administration & dosage;Glutathione Peroxidase - chemistry;HeLa Cells;Humans;nanocarriers;nanoparticles;Nanoparticles - administration & dosage;Nanoparticles - chemistry;Peptide Fragments - administration & dosage;Peptide Fragments - chemistry;porous media;Protein Denaturation;reactive oxygen species;Recombinant Fusion Proteins - administration & dosage;Recombinant Fusion Proteins - chemistry;silica;Silicon Dioxide - administration & dosage;Silicon Dioxide - chemistry;superoxide dismutase;Superoxide Dismutase - administration & dosage;Superoxide Dismutase - chemistry;tat Gene Products, Human Immunodeficiency Virus - administration & dosage;tat Gene Products, Human Immunodeficiency Virus - chemistry
Date:	2016-07-20
Issue Date:	2026-04-23 11:23:26 (UTC+8)
Publisher:	American Chemical Society;United States: American Chemical Society
Abstract:	摘要： Reactive oxygen species (ROS) are important factors in many clinical diseases. However, direct delivery of antioxidant enzymes into cells is difficult due to poor cell uptake. A proper design of delivery of enzymes by nanoparticles is very desirable for therapeutic purposes. To overcome the cell barrier problem, a designed mesoporous silica nanoparticle (MSN) system with attached TAT-fusion denatured enzyme for enhancing cell membrane penetration has been developed. Simultaneous delivery of two up–downstream antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase(GPx), reveals synergistic efficiency of ROS scavenging, compared to single antioxidant enzyme delivery. TAT peptide conjugation provided a facile nonendocytosis cell uptake and escape from endosome while moving and aggregating along the cytoskeleton that would allow them to be close to each other at the same time, resulting in the cellular antioxidation cascade reaction. The two-enzyme delivery shows a significant synergistic effect for protecting cells against ROS-induced cell damage and cell cycle arrest. The nanocarrier strategy for enzyme delivery demonstrates that intracellular anti-ROS cascade reactions could be regulated by multifunctional MSNs carrying image fluorophore and relevant antioxidation enzymes. 其他題名： ACS Appl. Mater. Interfaces 出版者： United States: American Chemical Society 出版日期： 2016-07-20 出處： ACS applied materials & interfaces, 2016-07, Vol.8 (28), p.17944-17954 資源來源： American Chemical Society Journals 版權： Copyright © 2016 American Chemical Society 識別號： ISSN: 1944-8244 識別號： ISSN: 1944-8252 識別號： EISSN: 1944-8252 識別號： DOI: 10.1021/acsami.6b05834 識別號： PMID: 27353012
Appears in Collections:	[Department of Optics and Photonics] journal & Dissertation

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