Development of chitosan oligosaccharide-modified gold nanorods for in vivo targeted delivery and noninvasive imaging by NIR irradiation

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題名:	Development of chitosan oligosaccharide-modified gold nanorods for in vivo targeted delivery and noninvasive imaging by NIR irradiation
作者:	簡汎清;Charan, Shobhit;Sanjiv, Kumar;Singh, Narendra;Chien, Fan-Ching;Chen, Yi-Fan;Nergui, Navchtsetseg Navchaa;Huang, Shih-Hsin;Kuo, Chiung Wen;Lee, Te-Chang;Chen, Peilin
貢獻者:	理學院光電科學與工程學系
關鍵詞:	Ammonium;Animals;Antibodies, Monoclonal - immunology;Antigen-Antibody Reactions;Carbohydrates;Cell Line, Tumor;Cell Survival - drug effects;Chitosan - chemistry;CHO Cells;Cricetinae;Dose-Response Relationship, Drug;Drug Delivery Systems;Epidermal growth factor;Gold;Gold - chemistry;Humans;Infrared radiation;Infrared Rays;Irradiation;Luminescent Measurements;Male;Medical imaging;Mice;Mice, Inbred BALB C;Mice, Nude;Molecular Probes - chemical synthesis;Molecular Probes - chemistry;Molecular Probes - pharmacology;Monoclonal antibodies;Nanomaterials;Nanotubes - chemistry;Oligosaccharides - chemistry;Photons;Receptor, Epidermal Growth Factor - genetics;Receptor, Epidermal Growth Factor - immunology;Structural engineering;Structure-Activity Relationship;Surface Properties;Tissue Distribution;Tumors
日期:	2012-11-21
上傳時間:	2026-04-23 11:26:47 (UTC+8)
出版者:	American Chemical Society;United States: American Chemical Society
摘要:	摘要： In the present study, we demonstrate the synthesis and applications of multifunctional gold nanorod-based probes for specific targeting and noninvasive imaging based on localized heating generated by gold nanorods after NIR irradiation. The structural design of the probe consists of MUA (11-mercaptoundecanoic acid)-capped gold nanorods covalently linked with low-molecular-weight chitosan oligosaccharide (M w ∼5000) via carbodiimide (EDC) coupling agent. This surface modification is performed for complete replacement of toxic CTAB (hexadecyltrimethyl-ammonium chloride) and acid-responsive delivery of gold nanorods in acidic environment as known to be present at tumor surrounding areas. The resulting chitosan oligosaccharide-modified gold nanorods (CO-GNRs) were further conjugated with tumor targeting monoclonal antibody against EGFR (epidermal growth factor receptor) to provide localized targeting functionality owing to the overexpression of EGFR in human oral adenosquamous carcinoma cell line CAL 27. Initial in vitro and in vivo toxicity assessments indicated that CO-GNRs did not induce any significant toxicity and are thus suitable for biological applications. Furthermore, selective targeting and accumulation of CO-GNRs were observed in vitro via two-photon luminescence imaging studies in CAL 27, which was also observed through in vivo targeting studies performed via NIR (near-infrared) laser irradiation in CAL 27 xenografts of BALB/c nude mice. Hence, the CO-GNRs that we have developed are biocompatible and nontoxic and can be a potential candidate for in vivo targeted delivery, noninvasive imaging based on localized hyperthermia, and photothermal-related therapies. 其他題名： Bioconjugate Chem 出版者： United States: American Chemical Society 出版日期： 2012-11-21 出處： Bioconjugate chemistry, 2012-11, Vol.23 (11), p.2173-2182 資源來源： American Chemical Society Journals (NTUSG) 版權： Copyright © 2012 American Chemical Society 版權： Copyright American Chemical Society Nov 21, 2012 識別號： ISSN: 1043-1802 識別號： ISSN: 1520-4812 識別號： EISSN: 1520-4812 識別號： DOI: 10.1021/bc3001276 識別號： PMID: 23030814
顯示於類別:	[光電科學與工程學系] 期刊論文

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