Dual-color dynamic tracking of GM-CSF receptors/JAK2 kinases signaling activation using temporal focusing multiphoton fluorescence excitation and astigmatic imaging

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/103301

Title:	Dual-color dynamic tracking of GM-CSF receptors/JAK2 kinases signaling activation using temporal focusing multiphoton fluorescence excitation and astigmatic imaging
Authors:	簡汎清;Chien, Fan-Ching;Liu, Chun-Shan;Lien, Chi-Hsiang;Dai, Yang-Hong;Yen, Jeffrey Jong-Young
Contributors:	理學院光電科學與工程學系
Keywords:	Enzyme Activation;Equipment Design;Equipment Failure Analysis;HeLa Cells;Humans;Image Enhancement - instrumentation;Janus Kinase 2 - metabolism;Lenses;Lighting - instrumentation;Microscopy, Fluorescence, Multiphoton - instrumentation;Molecular Imaging - instrumentation;Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - metabolism;Reproducibility of Results;Sensitivity and Specificity;Signal Transduction - physiology
Date:	2015-11-30
Issue Date:	2026-04-23 11:27:29 (UTC+8)
Publisher:	United States
Abstract:	摘要： The dual-color dynamic particle tracking approach that uses temporal focusing multiphoton fluorescence excitation and two-channel astigmatic imaging is utilized to track molecular trajectories in three dimensions to explore molecular interactions. Images of two fluorophores were obtained to extract their positions by optical sectioning excitation using a fast temporal focusing multiphoton excitation microscope (TFMPEM) and by the simultaneous collection of data in two channels. The presented pair of cylindrical lenses, which was used to adjust the astigmatism effect with the minimum shifting of the imaging plane, was more feasible and flexible than single cylindrical lens for aligning two separate detection channels in astigmatic imaging. The lateral and axial positioning resolutions were observed to be approximately 9-13 nm and 23-30 nm respectively, for the two fluorescence channels. The dynamic movement and binding behavior of clusters of GM-CSF receptors and JAK2 kinases in HeLa cells in the presence of GM-CSF ligands were observed. Therefore, the proposed dual-color tracking strategy is useful for the dynamic study of molecular interactions in living specimens with a fast frame rate, less photobleaching, better penetration depth, and minimum optical trapping force. 其他題名： Opt Express 出版者： United States 出版日期： 2015-11-30 出處： Optics express, 2015-11, Vol.23 (24), p.30943-30955 資源來源： Optica Publishing Group Journals 識別號： ISSN: 1094-4087 識別號： EISSN: 1094-4087 識別號： DOI: 10.1364/OE.23.030943 識別號： PMID: 26698726
Appears in Collections:	[Department of Optics and Photonics] journal & Dissertation

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