MDPI Multidisciplinary Digital Publishing Institute;Switzerland: MDPI AG
摘要:
摘要： Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0–250 UL−1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment. 其他題名： Biosensors (Basel) 出版者： Switzerland: MDPI AG 出版日期： 2012-05-29 出處： Biosensors (Basel), 2012-05, Vol.2 (2), p.234-244 資源來源： Publicly Available Content Database (PQ_SDU_P3) 版權： Copyright MDPI AG 2012 版權： 2012 by the authors; licensee MDPI, Basel, Switzerland. 2012 識別號： ISSN: 2079-6374 識別號： EISSN: 2079-6374 識別號： DOI: 10.3390/bios2020234 識別號： PMID: 25585713
