Improvement of carbon tetrachloride-induced acute hepatic failure by transplantation of induced pluripotent stem cells without reprogramming factor c-Myc

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/103580

Title:	Improvement of carbon tetrachloride-induced acute hepatic failure by transplantation of induced pluripotent stem cells without reprogramming factor c-Myc
Authors:	陳思妤;Chang, Hua-Ming;Liao, Yi-Wen;Chiang, Chih-Hung;Chen, Yi-Jen;Lai, Ying-Hsiu;Chang, Yuh-Lih;Chen, Hen-Li;Jeng, Shaw-Yeu;Hsieh, Jung-Hung;Peng, Chi-Hsien;Li, Hsin-Yang;Chien, Yueh;Chen, Szu-Yu;Chen, Liang-Kung;Huo, Teh-Ia
Contributors:	理學院光電科學與工程學系
Keywords:	Animals;Antioxidants;Antioxidants - metabolism;Carbon;Carbon Tetrachloride;Carbon Tetrachloride - adverse effects;Cell death;Cell Differentiation;Cell Survival;Cell- and Tissue-Based Therapy;Cell- and Tissue-Based Therapy - methods;Cells, Cultured;Cellular Reprogramming;Chemical and Drug Induced Liver Injury;Chemical and Drug Induced Liver Injury - therapy;Disease Models, Animal;Enzymes;Hepatic Encephalopathy;Hepatic Encephalopathy - therapy;Hepatocytes;Hepatocytes - metabolism;Hospitals;induced pluripotent stem cell;c-Myc;carbon tetrachloride;hepatic failure;hepatic encephalopathy;Induced Pluripotent Stem Cells;Induced Pluripotent Stem Cells - transplantation;Kruppel-Like Factor 4;Kruppel-Like Transcription Factors;Kruppel-Like Transcription Factors - biosynthesis;Liver;Liver - pathology;Liver diseases;Liver Failure, Acute;Liver Failure, Acute - chemically induced;Liver Failure, Acute - therapy;Male;Mice;Mice, Inbred BALB C;Mice, Inbred C57BL;Mice, Nude;Octamer Transcription Factor-3;Octamer Transcription Factor-3 - biosynthesis;Proto-Oncogene Proteins c-myc;Proto-Oncogene Proteins c-myc - deficiency;Proto-Oncogene Proteins c-myc - genetics;Reactive Oxygen Species;Reactive Oxygen Species - metabolism;SOXB1 Transcription Factors;SOXB1 Transcription Factors - biosynthesis;Stem cells
Date:	2012-03-01
Issue Date:	2026-04-23 11:33:15 (UTC+8)
Publisher:	MDPI Multidisciplinary Digital Publishing Institute;Switzerland: MDPI AG
Abstract:	摘要： The only curative treatment for hepatic failure is liver transplantation. Unfortunately, this treatment has several major limitations, as for example donor organ shortage. A previous report demonstrated that transplantation of induced pluripotent stem cells without reprogramming factor c-Myc (3-genes iPSCs) attenuates thioacetamide-induced hepatic failure with minimal incidence of tumorigenicity. In this study, we investigated whether 3-genes iPSC transplantation is capable of rescuing carbon tetrachloride (CCl4)-induced fulminant hepatic failure and hepatic encephalopathy in mice. Firstly, we demonstrated that 3-genes iPSCs possess the capacity to differentiate into hepatocyte-like cells (iPSC-Heps) that exhibit biological functions and express various hepatic specific markers. 3-genes iPSCs also exhibited several antioxidant enzymes that prevented CCl4-induced reactive oxygen species production and cell death. Intraperitoneal transplantation of either 3-genes iPSCs or 3-genes iPSC-Heps significantly reduced hepatic necrotic areas, improved hepatic functions, and survival rate in CCl4-treated mice. CCl4-induced hepatic encephalopathy was also improved by 3-genes iPSC transplantation. Hoechst staining confirmed the successful engraftment of both 3-genes iPSCs and 3-genes iPSC-Heps, indicating the homing properties of these cells. The most pronounced hepatoprotective effect of iPSCs appeared to originate from the highest antioxidant activity of 3-gene iPSCs among all transplanted cells. In summary, our findings demonstrated that 3-genes iPSCs serve as an available cell source for the treatment of an experimental model of acute liver diseases. 其他題名： Int J Mol Sci 出版者： Switzerland: MDPI AG 出版日期： 2012-03-16 出處： International Journal of Molecular Sciences, 2012-03, Vol.13 (3), p.3598-3617 資源來源： Publicly available content database 版權： Copyright MDPI AG 2012 版權： 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. 2012 識別號： ISSN: 1422-0067 識別號： ISSN: 1661-6596 識別號： EISSN: 1422-0067 識別號： DOI: 10.3390/ijms13033598 識別號： PMID: 22489170
Appears in Collections:	[Department of Optics and Photonics] journal & Dissertation

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