Local pH tracking in living cells

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/103713

Title:	Local pH tracking in living cells
Authors:	簡汎清;Tsou, Chieh-Jui;Hsia, Chih-Hao;Chu, Jia-Yin;Hung, Yann;Chen, Yi-Ping;Chien, Fan-Ching;Chou, Keng C;Chen, Peilin;Mou, Chung-Yuan
Contributors:	理學院光電科學與工程學系
Date:	2015-03-07
Issue Date:	2026-04-23 11:36:02 (UTC+8)
Publisher:	Royal Society of Chemistry
Abstract:	摘要： Continuous and simultaneous 3D single-particle movement and local pH detection in HeLa cells were demonstrated for the first time by combining fluorescent mesoporous silica nanoparticles (FMSNs) and a single-particle tracking (SPT) technique with a precision of ∼10 nm. FMSNs, synthesized by the co-condensation of both pH-sensitive and reference dyes with a silica/surfactant source, allow long-term reliable ratiometric pH measurements with a precision better than 0.3 pH unit because of their excellent brightness and stability. pH variation in the surrounding area of FMSNs during endocytosis was monitored in real-time. Acidification and low mobility of FMSNs were observed at the early endocytic stage, whereas basification and high mobility of FMSNs were observed at the late stage. Our results indicate that it is possible to monitor local pH changes in the environments surrounding nanoparticles during the cellular uptake process of FMSNs, which provides much needed information for designing an efficient drug delivery nanosystem. Simultaneous 3D single-particle tracking and local pH detection in living cells was achieved with ratiometric fluorescent mesoporous silica nanoparticles. 出版日期： 2015-02-19 識別號： ISSN: 2040-3364 識別號： EISSN: 2040-3372 識別號： DOI: 10.1039/c4nr06545j
Appears in Collections:	[Department of Optics and Photonics] journal & Dissertation

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