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    Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/103803


    Title: Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution
    Authors: 陳思妤;Chen, Szu-Yu;Lu, Chiao-Sheng;Yeh, Chia-Hua
    Contributors: 理學院光電科學與工程學系
    Keywords: Microscopy
    Date: 2014-01-01
    Issue Date: 2026-04-23 11:37:53 (UTC+8)
    Publisher: The Optical Society;United States: Optical Society of America
    Abstract: 摘要: A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.
    其他題名: Biomed Opt Express
    出版者: United States: Optical Society of America
    出版日期: 2014-02-01
    出處: Biomedical optics express, 2014-02, Vol.5 (2), p.338-347
    資源來源: Optica Publishing Group Journals
    版權: 2014 Optical Society of America 2014 Optical Society of America
    識別號: ISSN: 2156-7085
    識別號: EISSN: 2156-7085
    識別號: DOI: 10.1364/BOE.5.000338
    識別號: PMID: 24575331
    Appears in Collections:[Department of Optics and Photonics] journal & Dissertation

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