Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/103807

Title:	Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device
Authors:	簡汎清;Cheng, Li-Chung;Lien, Chi-Hsiang;Da Sie, Yong;Hu, Yvonne Yuling;Lin, Chun-Yu;Chien, Fan-Ching;Xu, Chris;Dong, Chen Yuan;Chen, Shean-Jen
Contributors:	理學院光電科學與工程學系
Date:	2014-08-01
Issue Date:	2026-04-23 11:37:57 (UTC+8)
Publisher:	The Optical Society;United States: Optical Society of America
Abstract:	摘要： In this study, the light diffraction of temporal focusing multiphoton excitation microscopy (TFMPEM) and the excitation patterning of nonlinear structured-illumination microscopy (NSIM) can be simultaneously and accurately implemented via a single high-resolution digital micromirror device. The lateral and axial spatial resolutions of the TFMPEM are remarkably improved through the second-order NSIM and projected structured light, respectively. The experimental results demonstrate that the lateral and axial resolutions are enhanced from 397 nm to 168 nm (2.4-fold) and from 2.33 μm to 1.22 μm (1.9-fold), respectively, in full width at the half maximum. Furthermore, a three-dimensionally rendered image of a cytoskeleton cell featuring ~25 nm microtubules is improved, with other microtubules at a distance near the lateral resolution of 168 nm also able to be distinguished. 其他題名： Biomed Opt Express 出版者： United States: Optical Society of America 出版日期： 2014-08-01 出處： Biomedical optics express, 2014-08, Vol.5 (8), p.2526-2536 資源來源： Optica Publishing Group Journals 版權： 2014 Optical Society of America 2014 Optical Society of America 識別號： ISSN: 2156-7085 識別號： EISSN: 2156-7085 識別號： DOI: 10.1364/BOE.5.002526 識別號： PMID: 25136483
Appears in Collections:	[Department of Optics and Photonics] journal & Dissertation

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