A biomimetic honeycomb-like scaffold prepared by flow-focusing technology for cartilage regeneration

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/103923

Title:	A biomimetic honeycomb-like scaffold prepared by flow-focusing technology for cartilage regeneration
Authors:	林耿慧;Wang, Chen-Chie;Yang, Kai-Chiang;Lin, Keng-Hui;Wu, Chang-Chin;Liu, Yen-Liang;Lin, Feng-Huei;Chen, Ing-Ho
Contributors:	理學院物理學系
Keywords:	Aggrecans;Aggrecans - biosynthesis;Animals;Biomimetics;Cartilage;Cartilage - growth & development;cartilage tissue engineering;Cell Proliferation;Cell Survival;Chondrocytes;Chondrocytes - physiology;Collagen;Collagen Type II;Collagen Type II - biosynthesis;Collagens;flow-focusing;gelatin;Gelatins;Gene expression;Gene Expression Profiling;Glycosaminoglycans;Glycosaminoglycans - metabolism;Guided Tissue Regeneration;Guided Tissue Regeneration - methods;Immunohistochemistry;microfluidic;Microfluidics;Microfluidics - instrumentation;Microfluidics - methods;Polymerase chain reaction;Porosity;Protein expression;Ribonucleic acid;RNA;scaffold;Scaffolds;Survival;Swine;Tissue engineering;Tissue Scaffolds;Tissue Scaffolds - chemistry
Date:	2014-11-01
Issue Date:	2026-04-23 11:39:58 (UTC+8)
Publisher:	Wiley-VCH Verlag;United States: Blackwell Publishing Ltd
Abstract:	摘要： ABSTRACT<jats:label />A tissue engineering chondrocytes/scaffold construct provides a promise to cartilage regeneration. The architecture of a scaffold such as interconnections, porosities, and pore sizes influences the fates of seeding cells including gene expression, survival, migration, proliferation, and differentiation thus may determine the success of this approach. Scaffolds of highly ordered and uniform structures are desirable to control cellular behaviors. In this study, a newly designed microfluidic device based on flow‐focusing geometry was developed to fabricate gelatin scaffolds of ordered pores. In comparison with random foam scaffolds made by the conventional freeze‐dried method, honeycomb‐like scaffolds exhibit higher swelling ratio, porosity, and comparable compressive strength. In addition, chondrocytes grown in the honeycomb‐like scaffolds had good cell viability, survival rate, glycosaminoglycans production, and a better proliferation than ones in freeze‐dried scaffolds. Real‐time PCR analysis showed that the mRNA expressions of aggrecan and collagen type II were up‐regulated when chondrocytes cultured in honeycomb‐like scaffolds rather than cells cultured as monolayer fashion. Oppositely, chondrocytes expressed collagen type II as monolayer culture when seeded in freeze‐dried scaffolds. Histologic examinations revealed that cells produced proteoglycan and distributed uniformly in honeycomb‐like scaffolds. Immunostaining showed protein expression of S‐100 and collagen type II but negative for collagen type I and X, which represents the chondrocytes maintained normal phenotype. In conclusion, a highly ordered and honeycomb‐like scaffold shows superior performance in cartilage tissue engineering. Biotechnol. Bioeng. 2014;111: 2338–2348. © 2014 Wiley Periodicals, Inc. 其他題名： Biotechnol. Bioeng 出版者： United States: Blackwell Publishing Ltd 出版日期： 2014-11 出處： Biotechnology and Bioengineering, 2014-11, Vol.111 (11), p.2338-2348 資源來源： Wiley Online Library 版權： 2014 Wiley Periodicals, Inc. 版權： Copyright John Wiley and Sons, Limited Nov 2014 識別號： ISSN: 0006-3592 識別號： ISSN: 1097-0290 識別號： EISSN: 1097-0290 識別號： DOI: 10.1002/bit.25295 識別號： PMID: 24895237 識別號： CODEN: BIBIAU
Appears in Collections:	[Department of Physics] journal & Dissertation

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