Pico-projector-based optical sectioning microscopy for 3D chlorophyll fluorescence imaging of mesophyll cells

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/104001

Title:	Pico-projector-based optical sectioning microscopy for 3D chlorophyll fluorescence imaging of mesophyll cells
Authors:	陳思妤;Chen, Szu-Yu;Hsu, Yu John;Yeh, Chia-Hua;Chen, S-Wei;Chung, Chien-Han
Contributors:	理學院光電科學與工程學系
Keywords:	chlorophyll;Chlorophylls;digital micromirror device;Fluorescence;fluorescence imaging;Illumination;Imaging;Modulation;Optical sectioning;Pattern generation;structured illumination;Three dimensional
Date:	2015-02-20
Issue Date:	2026-04-23 11:41:33 (UTC+8)
Publisher:	IOP Publishing Ltd.;IOP Publishing
Abstract:	摘要： A pico-projector-based optical sectioning microscope (POSM) was constructed using a pico-projector to generate structured illumination patterns. A net rate of 5.8 × 106 pixel s and sub-micron spatial resolution in three-dimensions (3D) were achieved. Based on the pico-projector's flexibility in pattern generation, the characteristics of POSM with different modulation periods and at different imaging depths were measured and discussed. With the application of different modulation periods, 3D chlorophyll fluorescence imaging of mesophyll cells was carried out in freshly plucked leaves of four species without sectioning or staining. For each leaf, an average penetration depth of 120 m was achieved. Increasing the modulation period along with the increment of imaging depth, optical sectioning images can be obtained with a compromise between the axial resolution and signal-to-noise ratio. After ∼30 min imaging on the same area, photodamage was hardly observed. Taking the advantages of high speed and low damages of POSM, the investigation of the dynamic fluorescence responses to temperature changes was performed under three different treatment temperatures. The three embedded blue, green and red light-emitting diode light sources were applied to observe the responses of the leaves with different wavelength excitation. 其他題名： JOPT 其他題名： J. Opt 出版者： IOP Publishing 出版日期： 2015-03-01 出處： Journal of Optics, 2015-03, Vol.17 (3), p.35301-11 資源來源： Institute of Physics Journals 版權： 2015 IOP Publishing Ltd 識別號： ISSN: 2040-8978 識別號： EISSN: 2040-8986 識別號： EISSN: 1464-4258 識別號： DOI: 10.1088/2040-8978/17/3/035301 識別號： CODEN: JOOPCA
Appears in Collections:	[Department of Optics and Photonics] journal & Dissertation

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