Calibration of optical tweezers for in vivo force measurements: How do different approaches compare?

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题名:	Calibration of optical tweezers for in vivo force measurements: How do different approaches compare?
作者:	田溶根;Jun, Yonggun;Tripathy, Suvranta K.;Narayanareddy, Babu R.J.;Mattson-Hoss, Michelle K.;Gross, Steven P.
贡献者:	理學院物理學系
关键词:	Adenosine Triphosphate - metabolism;Calibration;Cells;HEK293 Cells;Humans;Hydrolysis;Kinesin - metabolism;Mechanical Phenomena;Optical properties;Optical Tweezers;Optics;Systems Biophysics;Temperature
日期:	2014-09-16
上传时间:	2026-04-23 11:44:18 (UTC+8)
出版者:	Cell Press;United States: Elsevier Inc
摘要:	摘要： There is significant interest in quantifying force production inside cells, but since conditions in vivo are less well controlled than those in vitro, in vivo measurements are challenging. In particular, the in vivo environment may vary locally as far as its optical properties, and the organelles manipulated by the optical trap frequently vary in size and shape. Several methods have been proposed to overcome these difficulties. We evaluate the relative merits of these methods and directly compare two of them, a refractive index matching method, and a light-momentum-change method. Since in vivo forces are frequently relatively high (e.g., can exceed 15 pN for lipid droplets), a high-power laser is employed. We discover that this high-powered trap induces local temperature changes, and we develop an approach to compensate for uncertainties in the magnitude of applied force due to such temperature variations. 其他題名： Biophys J 出版者： United States: Elsevier Inc 出版日期： 2014-09-16 出處： Biophysical Journal, 2014-09, Vol.107 (6), p.1474-1484 資源來源： Elsevier ScienceDirect Journals Complete ** 版權： 2014 Biophysical Society 版權： Copyright © 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved. 版權： Copyright Biophysical Society Sep 16, 2014 版權： 2014 by the Biophysical Society. 2014 Biophysical Society 識別號： ISSN: 0006-3495 識別號： ISSN: 1542-0086 識別號： EISSN: 1542-0086 識別號： DOI: 10.1016/j.bpj.2014.07.033 識別號： PMID: 25229154
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