Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold

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題名:	Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold
作者:	林耿慧;Wang, Chen-Chie;Yang, Kai-Chiang;Lin, Keng-Hui;Liu, Yen-Liang;Liu, Hwa-Chang;Lin, Feng-Huei
貢獻者:	理學院物理學系
關鍵詞:	Advanced Basic Science;Aggrecans - genetics;Alginate;Alginates - chemistry;Animals;cartilage;Cartilage - cytology;Cartilage - metabolism;Cartilage tissue engineering;Chondrocyte;chondrocytes;Chondrocytes - cytology;Chondrocytes - metabolism;collagen;Collagen Type II - genetics;Dentistry;gene expression regulation;Glucuronic Acid - chemistry;glycosaminoglycans;Hexuronic Acids - chemistry;Immunohistochemistry;in vitro culture;messenger RNA;Mice;Mice, SCID;osteoarthritis;phenotype;quantitative polymerase chain reaction;Real-Time Polymerase Chain Reaction;Scaffold;SCID mouse;severe combined immunodeficiency;surgery;swine;tissue engineering;Tissue Engineering - methods;Tissue Scaffolds - chemistry
日期:	2012-01-01
上傳時間:	2026-04-23 11:44:32 (UTC+8)
出版者:	Elsevier BV;Netherlands: Elsevier Ltd
摘要:	摘要： Tissue engineering for cartilage regeneration provides an alternative to surgery for degenerative osteoarthritis. Recently, a highly organized three-dimensional (3D) alginate scaffold was prepared using a microfluidic device; this scaffold is effective for chondrocyte culture in vitro. The performance of this scaffold was further demonstrated; an alginate scaffold seeded with porcine chondrocytes was implanted in the dorsal subcutaneous site of SCID mice. The recipients were sacrificed at 2, 4, and 6 weeks after transplantation. The grafted implants retrieved from the subcutaneous site were analyzed with histologic examinations. Real-time PCR was used to identify the gene expression patterns of the chondrocytes. The hematoxylin and eosin staining showed that the chondrocytes survived normally in SCID mice; cartilage-like structures were formed after 4 weeks implantation. Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100. On the other hand, the cells were negative for type I and type X collagen staining. PCR showed that the mRNA expressions of aggrecan and type II collagen were up-regulated at weeks two and four, while type I and type X collagen were down-regulated during the study period. In summary, this highly organized 3D alginate scaffold provided a suitable environment and maintained functional phenotypes for chondrocytes in this animal study. 其他題名： Biomaterials 出版者： Netherlands: Elsevier Ltd 出版日期： 2012-01-01 出處： Biomaterials, 2012-01, Vol.33 (1), p.120-127 版權： 2011 Elsevier Ltd 版權： Elsevier Ltd 版權： Copyright Â© 2011 Elsevier Ltd. All rights reserved. 識別號： ISSN: 0142-9612 識別號： ISSN: 1878-5905 識別號： EISSN: 1878-5905 識別號： DOI: 10.1016/j.biomaterials.2011.09.042 識別號： PMID: 21982587
顯示於類別:	[物理學系] 期刊論文

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