MDPI Multidisciplinary Digital Publishing Institute;Switzerland: MDPI AG
Abstract:
摘要： A single-use disposable in vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. A three-electrode configuration electrochemical biosensor consisted of 10-nm-thin gold film working and counter electrodes and a thick-film printed Ag/AgCl reference electrode was fabricated on a polyethylene terephthalate (PET) substrate. Micro-fabrication techniques including sputtering vapor deposition and thick-film printing were used to fabricate the biosensor. This was a roll-to-roll cost-effective manufacturing process making the single-use disposable in vitro HbA1c biosensor a reality. Self-assembled monolayers of 3-Mercaptopropionic acid (MPA) were employed to covalently immobilize anti-HbA1c on the surface of gold electrodes. Electrochemical impedance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) confirmed the excellent coverage of MPA-SAM and the upward orientation of carboxylic groups. The hindering effect of HbA1c on the ferricyanide/ferrocyanide electron transfer reaction was exploited as the HbA1c detection mechanism. The biosensor showed a linear range of 7.5–20 µg/mL of HbA1c in 0.1 M PBS. Using undiluted human serum as the test medium, the biosensor presented an excellent linear behavior (R2 = 0.999) in the range of 0.1–0.25 mg/mL of HbA1c. The potential application of this biosensor for in vitro measurement of HbA1c for diabetic management was demonstrated. 其他題名： Sensors (Basel) 出版者： Switzerland: MDPI AG 出版日期： 2016-07-01 出處： Sensors, 2016-07, Vol.16 (7), p.1024--1024 資源來源： Publicly Available Content Database 版權： Copyright MDPI AG 2016 版權： 2016 by the authors; licensee MDPI, Basel, Switzerland. 2016 識別號： ISSN: 1424-8220 識別號： EISSN: 1424-8220 識別號： DOI: 10.3390/s16071024 識別號： PMID: 27376299
