CK2 activates kinesin via induction of a conformational change

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題名:	CK2 activates kinesin via induction of a conformational change
作者:	田溶根;Mattson-Hoss, Michelle K.;Niitani, Yamato;Gordon, Elizabeth A.;Jun, Yonggun;Bardwell, Lee;Tomishige, Michio;Gross, Steven P.
貢獻者:	理學院物理學系
關鍵詞:	Adenosine Diphosphate;Adenosine Diphosphate - metabolism;Adenosine triphosphatases;Adenosine Triphosphate;Adenosine Triphosphate - metabolism;Animals;Biological Sciences;Cancer;cargo travel;Casein Kinase II;Casein Kinase II - chemistry;Casein Kinase II - metabolism;Cells;Cellular biology;dissociation;Enzyme Activation;Enzyme Activation - physiology;Freight;Gels;Humans;Ice;Inactivation;Kinases;Kinesin;Kinesin - chemistry;Kinesin - metabolism;Kinesins;Microtubules;Microtubules - physiology;Models, Molecular;Molecular;Motors;multiple-motor transport;non-specific serine/threonine protein kinase;Nucleotides;Pelleted feed;Physiological regulation;Population aging;Protein Structure;Protein Structure, Tertiary;Proteins;Repositioning;Signal Transduction;Signal Transduction - physiology;Tertiary;transport regulation
日期:	2014-05-13
上傳時間:	2026-04-23 11:45:22 (UTC+8)
出版者:	National Academy of Sciences;United States: National Academy of Sciences
摘要:	摘要： Kinesin is the canonical plus-end microtubule motor and has been the focus of intense study since its discovery in 1985. We previously demonstrated a time-dependent inactivation of kinesin in vitro that was fully reversible by the addition of purified casein kinase 2 (CK2) and showed that this inactivation/reactivation pathway was relevant in cells. Here we show that kinesin inactivation results from a conformational change that causes the neck linker to be positioned closer to the motor domain. Furthermore, we show that treatment of kinesin with CK2 prevents and reverses this repositioning. Finally, we demonstrate that CK2 treatment facilitates ADP dissociation from the motor, resulting in a nucleotide-free state that promotes microtubule binding. Thus, we propose that kinesin inactivation results from neck-linker repositioning and that CK2-mediated reactivation results from CK2’s dual ability to reverse this repositioning and to promote ADP release. 其他題名： Proc Natl Acad Sci U S A 出版者： United States: National Academy of Sciences 出版日期： 2014-05-13 出處： Proceedings of the National Academy of Sciences, 2014-05, Vol.111 (19), p.7000-7005 資源來源： JSTOR (Life Sciences) 版權： copyright © 1993–2008 National Academy of Sciences of the United States of America 版權： Copyright National Academy of Sciences May 13, 2014 識別號： ISSN: 0027-8424 識別號： ISSN: 1091-6490 識別號： EISSN: 1091-6490 識別號： DOI: 10.1073/pnas.1321419111 識別號： PMID: 24782540
顯示於類別:	[物理學系] 期刊論文

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