Surface charge effect in intracellular localization of mesoporous silica nanoparticles as probed by fluorescent ratiometric pH imaging

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/104287

Title:	Surface charge effect in intracellular localization of mesoporous silica nanoparticles as probed by fluorescent ratiometric pH imaging
Authors:	簡汎清;Chen, Yiping;Chen, Hsueh-An;Hung, Yann;Chien, Fan-Ching;Chen, Peilin;Mou, Chung-Yuan
Contributors:	理學院光電科學與工程學系
Keywords:	cytosol;endosomes;fluorescence;human cell lines;image analysis;monitoring;nanocarriers;nanoparticles;porous media;silica;tissues
Date:	2012-02-07
Issue Date:	2026-04-23 11:47:21 (UTC+8)
Publisher:	Royal Society of Chemistry
Abstract:	摘要： Mesoporous silica nanoparticles (MSNs) have received intensive recent interest as multi-functional nanocarriers for biological cells and tissues. Their cell uptake and intracellular distribution depends on the surface charge they carry. We develop a new approach for monitoring their uptake and intracellular localization by measuring the local pH value of the MSNs. We synthesized a ratiometric pH-sensor loaded with mesoporous silica nanoparticles that can map the location of the MSN through the site pH in HeLa cells. Positively charged MSN-TA was found to locate in higher pH regions which are identified as cytosol mostly. On the other hand, negatively charged MSN-PPs were trapped in acidic endosomes. Evidence is given for direct penetration of membranes through a charge-mediated membrane–nanoparticle interaction mechanism. 出版日期： 2012-02-07 出處： RSC advances, 2012-02, Vol.2 (3 p.968-973), p.968-973 資源來源： Royal Society of Chemistry 識別號： ISSN: 2046-2069 識別號： EISSN: 2046-2069 識別號： DOI: 10.1039/c1ra00586c
Appears in Collections:	[Department of Optics and Photonics] journal & Dissertation

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