Use of liposomal amplifiers in total internal reflection fluorescence fiber-optic biosensors for protein detection

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題名:	Use of liposomal amplifiers in total internal reflection fluorescence fiber-optic biosensors for protein detection
作者:	陳啟昌;Chang, Ying-Feng;Fu, Chen;Chen, Yi-Ting;Fang-Ju Jou, Amily;Chen, Chii-Chang;Chou, Chien;Annie Ho, Ja-an
貢獻者:	理學院光電科學與工程學系
關鍵詞:	Amplifiers;Biosensing Techniques - instrumentation;Biosensors;cost effectiveness;detection limit;Equipment Design;Equipment Failure Analysis;Evanescent wave-excited fluorescence;Excitation;Fiber Optic Technology - instrumentation;fiber optics;Fiber-optic biosensors;Fluorescence;Format;Immunoassay - instrumentation;immunoassays;immunoglobulin G;Immunoglobulin G - analysis;Liposomes;Liposomes - chemistry;polyclonal antibodies;Protein Array Analysis - instrumentation;Protein detection;Proteins;Reflection;Spectrometry, Fluorescence - instrumentation;Target detection
日期:	2016-03-15
上傳時間:	2026-04-23 11:50:05 (UTC+8)
出版者:	Elsevier Ltd.;England: Elsevier B.V
摘要:	摘要： Evanescent-wave excited fluorescence technology has been demonstrated to enhance sensitivity and reduce matrix effects, making it suitable for biosensor development. In this study, we developed a liposome-based, total internal reflection fluorescence, fiber-optic biosensor (TIRF-FOB) for protein detection, which integrates a liposomal amplifier and sandwich immunoassay format with TIRF-FOB. In addition, the antibody-tagged and fluorophore-entrapped liposomes for heterogeneous detection of target molecules were designed and synthesized. This biosensor successfully detected the target protein (model analyzed here is IgG) with a limit of detection (LOD) of 2.0 attomoles for the target protein (equivalent to 2.0pg/mL of protein presented in 150μL of sample solution). The features of this ultra-sensitive liposomal TIRF-FOB are (i) fluorescence is excited via evanescent waves and amplified via liposomes; (ii) the use of two polyclonal antibodies in the sandwich assay format increases the specificity and lowers the cost of our assay. Based on the exceptional detection sensitivity and cost-effectiveness, we believe that the proposed biosensor has great potential as a practical, clinical diagnostic tool in the near future. •Evanescent-wave excited fluorescence technology was used to develop a new sensor.•Liposomal amplifiers and sandwich immunoassay format were integrated with TIRF-FOB.•Ultra-sensitivity of TIRF-FOB was achieved by liposomal signal amplification.•The proposed biosensor has great potential as a practical, clinical diagnostic tool. 其他題名： Biosens Bioelectron 出版者： England: Elsevier B.V 出版日期： 2016-03-15 出處： Biosensors & bioelectronics, 2016-03, Vol.77, p.1201-1207 版權： 2015 Elsevier B.V. 版權： Copyright © 2015 Elsevier B.V. All rights reserved. 識別號： ISSN: 0956-5663 識別號： EISSN: 1873-4235 識別號： DOI: 10.1016/j.bios.2015.10.012 識別號： PMID: 26595485
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