指叉電極檢測系統以阻抗分析方式檢測老鼠纖維組織細胞L929的新陳代謝及增殖; Impedance analysis of metabolism and proliferation of mouse fibroblast cells L929 by a portable interdigitated microelectrodes detection system

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题名:	指叉電極檢測系統以阻抗分析方式檢測老鼠纖維組織細胞L929的新陳代謝及增殖;Impedance analysis of metabolism and proliferation of mouse fibroblast cells L929 by a portable interdigitated microelectrodes detection system
作者:	蔡孟廷;Meng-ting Tsai
贡献者:	電機工程研究所
关键词:	阻抗量測;細胞培養;電雙層;指叉電極;double layer;cell culture;impedance measurement;interdigitated electrode
日期:	2009-07-09
上传时间:	2009-09-22 12:17:21 (UTC+8)
出版者:	國立中央大學圖書館
摘要:	本研究利用指叉型電極來檢測老鼠纖維母細胞L929生長代謝及分裂後時阻抗的變化。指叉型電極利用微影製程的方式製作，電極尺寸為500um x 8um x 50nm。本研究將L929附著於生物感測晶片上，然後量測不同濃度及不同生長情況下阻抗變化情況。在進行阻抗量測前，會先於感測晶片表面上一層Poly-L-lysine使細胞更容易貼附。初步結果為不同細胞數量相對造成不同的阻抗變化，其原因為細胞代謝物會影響電雙層電容的變化。為了達到方便及快速檢測，本研究利用微控制器、生物感測晶片及阻抗量測元件 AD5933來發展簡易的阻抗量測系統。利用此阻抗量測系統初步可以觀察到在不同細胞濃度下其代謝對阻抗造成的變化。因此，此生物檢測系統利用阻抗量測方式確實對於細胞代謝的量測提供一個有效的分析方式。 Interdigitated microelectrodes were used to monitor the growth of L929 cells during metabolism and proliferation. The interdigitated microelectrodes were fabricated with microfabrication technology with 8μm of width, 8μm of gap, and 500μm of length for each electrode. In this study, the mouse fibroblast cells L929 was cultured on the electrodes surface with pre-coated poly-L-lysine. The impedance change in the interdigitated electrode pair was measured during cells adhesion, spreading, and proliferation. The experimental results show that cells concentration increase could affect the impedance change because the cell’s metabolite correlated to the double layer capacitance between the electrodes and the cells. Further, to develop a portable and fast detection device, we built an impedance detection system using a microprocessor, the impedance convertor AD5933, and the interdigitated microelectrodes chip. Using this impedance detection system can prove to observe metabolism of different concentration for cells growth stage. Thus, this cell-growth monitor system by impedance measurement provides a useful analytical method for cells metabolism.
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