Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

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題名:	Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds
作者:	林耿慧;Sun, Yung-Shin;Peng, Shih-Wei;Lin, Keng-Hui;Cheng, Ji-Yen
貢獻者:	理學院物理學系
關鍵詞:	Regular
日期:	2012-03-02
上傳時間:	2026-04-23 11:52:48 (UTC+8)
出版者:	American Institute of Physics;United States: AIP Publishing
摘要:	摘要： In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D scaffold consists of ordered arrays of uniform spherical pores in gelatin. We found that cell morphology in the 3D scaffold was different from that in 2D substrate. Next, we applied a direct current electric field (EF) of 338 mV/mm through the scaffold for the study of cells’ migration within. We measured the migration directedness and speed of different lung cancer cell lines, CL1-0, CL1-5, and A549, and compared with those examined in 2D gelatin-coated and bare substrates. The migration direction is the same for all conditions but there are clear differences in cell morphology, directedness, and migration speed under EF. Our results demonstrate cell migration under EF is different in 2D and 3D environments and possibly due to different cell morphology and/or substrate stiffness. 其他題名： Biomicrofluidics 出版者： United States: AIP Publishing 出版日期： 2012-03-01 出處： Biomicrofluidics, 2012-03, Vol.6 (1), p.014102-014102-14 資源來源： AIP Journals (American Institute of Physics) 版權： American Institute of Physics 版權： 2012 American Institute of Physics 版權： Copyright © 2012 American Institute of Physics 2012 American Institute of Physics 識別號： ISSN: 1932-1058 識別號： EISSN: 1932-1058 識別號： DOI: 10.1063/1.3671399 識別號： PMID: 22288000 識別號： CODEN: BIOMGB
顯示於類別:	[物理學系] 期刊論文

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