Expandable Scaffold Improves Integration of Tissue-Engineered Cartilage: An in Vivo Study in a Rabbit Model

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/104677

Title:	Expandable Scaffold Improves Integration of Tissue-Engineered Cartilage: An in Vivo Study in a Rabbit Model
Authors:	林耿慧;Wang, Chen-Chie;Yang, Kai-Chiang;Lin, Keng-Hui;Liu, Yen-Liang;Yang, Ya-Ting;Kuo, Tzong-Fu;Chen, Ing-Ho
Contributors:	理學院物理學系
Keywords:	Animals;Biomedical materials;Cartilage;Cartilage, Articular - physiology;Cellular biology;Compressive Strength;Microbubbles;Models, Animal;Original Articles;Rabbits;Regeneration;Tissue engineering;Tissue Engineering - methods;Tissue Scaffolds - chemistry;Wound Healing
Date:	2016-06-01
Issue Date:	2026-04-23 11:55:21 (UTC+8)
Publisher:	Mary Ann Liebert Inc.;Los Angeles, CA: Mary Ann Liebert, Inc
Abstract:	摘要： One of the major limitations of tissue-engineered cartilage is poor integration of chondrocytes and scaffold structures with recipient tissue. To overcome this limitation, an expandable scaffold with a honeycomb-like structure has been developed using microfluidic technology. In this study, we evaluated the performance of this expandable gelatin scaffold seeded with rabbit chondrocytes in vivo . The chondrocyte/scaffold constructs were implanted into regions of surgically introduced cylindrical osteochondral defects in rabbit femoral condyles. At 2, 4, and 6 months postsurgery, the implanted constructs were evaluated by gross and histological examinations. As expected, the osteochondral defects, which were untreated or transplanted with blank scaffolds, showed no signs of repair, whereas the defects transplanted with chondrocyte/scaffold constructs showed significant cartilage regeneration. Furthermore, the expandable scaffolds seeded with chondrocytes had more regenerated cartilage tissue and better integration with the recipient tissue than autologous chondrocyte implantation. Biomechanical tests revealed that the chondrocyte/scaffold group had the highest compressive strength among all groups at all three time points and endured a similar compressive force to normal cartilage after 6 months of implantation. Histological examinations revealed that the chondrocytes were distributed uniformly within the scaffolds, maintained a normal phenotype, and secreted functional components of the extracellular matrix. Histomorphometric assessment showed a remarkable total interface of up to 87% integration of the expandable scaffolds with the host tissue at 6 months postoperation. In conclusion, the expandable scaffolds improved chondrocyte/scaffold construct integration with the host tissue and were beneficial for cartilage repair. 其他題名： Tissue Eng Part A 出版者： Los Angeles, CA: Mary Ann Liebert, Inc 出版日期： 2016-06-01 出處： Tissue engineering. Part A, 2016-06, Vol.22 (11-12), p.873-884 版權： 2016, Mary Ann Liebert, Inc. 版權： Copyright 2016, Mary Ann Liebert, Inc. 版權： (©) Copyright 2016, Mary Ann Liebert, Inc. 識別號： ISSN: 1937-3341 識別號： EISSN: 1937-335X 識別號： DOI: 10.1089/ten.tea.2015.0510 識別號： PMID: 27193498
Appears in Collections:	[Department of Physics] journal & Dissertation

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