Springer Verlag;Berlin/Heidelberg: Springer Berlin Heidelberg
摘要:
摘要： Stem cell research grows rapidly in recent years due to its potential in clinical applications. Since the stem cell behavior is influenced by environmental cues, investigation of the stem cell differentiation under chemical as well as physical stimulation environment is important. Under traditional petri dish culturing conditions, directly applying physical stimulation on the cells is difficult. Microfluidic chip, on the other hand, provides a manageable platform to stimulate cells. In this research, we use microfluidic chip to culture human placenta-derived multipotent stem cells (PDMCs) and apply chemical and physical stimulations to the cells. PDMCs is a newly developed human stem cell source that are easier to access with less ethical issues compare to other human stem cells. This research shows that the microfluidic platform could provide adequate in vitro microenvironment that maintain pluripotency and proliferative potential for PDMCs culture. PDMCs were successfully differentiate to neuronal cells through chemical and physical–chemical stimulation on the microfluidic platform. Our results show that through physical shear stress stimulation, PDMCs can result in enhanced neuronal cell differentiation. 其他題名： Microfluid Nanofluid 出版者： Berlin/Heidelberg: Springer Berlin Heidelberg 出版日期： 2015-04-01 出處： Microfluidics and nanofluidics, 2015-04, Vol.18 (4), p.587-598 資源來源： Agricultural & Environmental Science Collection 版權： Springer-Verlag Berlin Heidelberg 2014 版權： Springer-Verlag Berlin Heidelberg 2015 識別號： ISSN: 1613-4982 識別號： EISSN: 1613-4990 識別號： DOI: 10.1007/s10404-014-1455-2
