Osteogenic differentiation of placenta-derived multipotent cells in vitro

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題名:	Osteogenic differentiation of placenta-derived multipotent cells in vitro
作者:	鍾志昂;Cheng, Chih-Chien;Chung, Chih-Ang;Su, Li-Chiu;Chien, Chih-Cheng;Cheng, Yu-Che
貢獻者:	工學院能源工程研究所
關鍵詞:	Alkaline Phosphatase - metabolism;Calcium - metabolism;Cell Differentiation - genetics;Cells, Cultured;Core Binding Factor Alpha 1 Subunit - genetics;differentiation;Female;Gene Expression;Humans;Internal Medicine;Multipotent Stem Cells - enzymology;Multipotent Stem Cells - physiology;Obstetrics and Gynecology;Osteocalcin - genetics;osteogenesis;Osteogenesis - genetics;Placenta;placenta-derived multipotent cells (PDMCs);Pregnancy;RNA, Messenger - metabolism;Up-Regulation
日期:	2014-01-01
上傳時間:	2026-04-23 12:01:45 (UTC+8)
出版者:	Elsevier Taiwan LLC;China (Republic : 1949- ): Elsevier B.V
摘要:	摘要： Stem cells offer great potential for clinical therapeutic use because of their ability to rejuvenate and to differentiate into numerous types of cells. We isolated multipotent cells from the human term placenta that were capable of differentiation into cells of all three germ layers. We examined the ability of these placenta-derived multipotent cells (PDMCs) to differentiate into osteoblasts (OBs) or OB-like cells. The PDMCs were treated with osteogenic medium (OM) consisting of dexamethasone, β-glycerol phosphate, and ascorbic acid. At sequential time intervals (0 day, 3 days, 6 days, 9 days, and 12 days) we measured several parameters. These included alkaline phosphatase (ALP) activity, alizarin red staining (ARS) to measure calcium deposition, messenger RNA (mRNA) expressions of osteogenesis-related transcription factor (Cbfa1), and calcium coordination protein (osteocalcin). These variables were used as indicators of PDMC osteodifferentiation. We showed that ALP activity in the early stage of differentiation and calcium deposition were both significantly increased in PDMCs after OM induction. Moreover, the Cbfa1 and osteocalcin gene expressions were upregulated. The results suggested that OM induced an osteodifferentiation potential in PDMCs. PDMC-derived osteocytes provide a useful model to evaluate the mechanisms of key biomolecules and bioengineering processes. 其他題名： Taiwan J Obstet Gynecol 出版者： China (Republic : 1949- ): Elsevier B.V 出版日期： 2014-06-01 出處： Taiwanese journal of obstetrics & gynecology, 2014-06, Vol.53 (2), p.187-192 資源來源： Elsevier ScienceDirect Journals Complete 版權： 2014 版權： Copyright © 2014. Published by Elsevier B.V. 識別號： ISSN: 1028-4559 識別號： ISSN: 1875-6263 識別號： EISSN: 1875-6263 識別號： DOI: 10.1016/j.tjog.2014.04.011 識別號： PMID: 25017264
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