Kinesin-1 translocation: Surprising differences between bovine brain and MCF7-derived microtubules

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题名:	Kinesin-1 translocation: Surprising differences between bovine brain and MCF7-derived microtubules
作者:	田溶根;Feizabadi, Mitra Shojania;Jun, Yonggun
贡献者:	理學院物理學系
关键词:	Animals;brain;Brain - cytology;Brain - metabolism;breast neoplasms;Cattle;Humans;Kinesin - metabolism;Kinesin-1 translocation;MCF-7 Cells;MCF7 cell–bovine brain cell;Microtubule;microtubules;Microtubules - metabolism;Movement;neoplasm cells;polymerization;swine;tubulin
日期:	2014-11-28
上传时间:	2026-04-23 12:08:02 (UTC+8)
出版者:	Academic Press Inc.;United States: Elsevier Inc
摘要:	摘要： •We characterized the in vitro translocation of kinesin-1 along MCF7 microtubules.•As compared to bovine microtubules, its run length along MCF7 microtubules is shorter.•As compared to bovine microtubules, its velocity is slightly altered. While there have been many single-molecule studies of kinesin-1, most have been done along microtubules purified from bovine or porcine brain, and relatively little is known about how variations in tubulin might alter motor function. Of particular interest is transport along microtubules polymerized from tubulin purified from MCF7 breast cancer cells, both because these cells are a heavily studied model system to help understand breast cancer, and also because the microtubules are already established to have interesting polymerization/stability differences from bovine tubulin, suggesting that perhaps transport along them is also different. Thus, we carried out paired experiments to allow direct comparison of in vitro kinesin-1 translocation along microtubules polymerized from either human breast cancer cells (MCF7) or microtubules from bovine brain. We found surprising differences: on MCF7 microtubules, kinesin-1’s processivity is significantly reduced, although its velocity is only slightly altered. 其他題名： Biochem Biophys Res Commun 出版者： United States: Elsevier Inc 出版日期： 2014-11-28 出處： Biochemical and biophysical research communications, 2014-11, Vol.454 (4), p.543-546 版權： 2014 Elsevier Inc. 版權： Copyright © 2014 Elsevier Inc. All rights reserved. 識別號： ISSN: 0006-291X 識別號： ISSN: 1090-2104 識別號： EISSN: 1090-2104 識別號： DOI: 10.1016/j.bbrc.2014.10.119 識別號： PMID: 25450690
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