Matrix dimensionality and stiffness cooperatively regulate osteogenesis of mesenchymal stromal cells

NCU Institutional Repository > 理學院 > 物理學系 > 期刊論文 > Item 987654321/105157

請使用永久網址來引用或連結此文件: https://ir.lib.ncu.edu.tw/handle/987654321/105157

題名:	Matrix dimensionality and stiffness cooperatively regulate osteogenesis of mesenchymal stromal cells
作者:	林耿慧;Hsieh, Wen-Ting;Liu, Yi-Shiuan;Lee, Yi-hsuan;Rimando, Marilyn G.;Lin, Keng-hui;Lee, Oscar K.
貢獻者:	理學院物理學系
關鍵詞:	Acrylic Resins - pharmacology;Actins - metabolism;Biocompatibility;Biomedical materials;Cell Differentiation - drug effects;Cell Differentiation - genetics;Cell Proliferation - drug effects;Cell Shape - drug effects;Cellular;Extracellular Matrix - metabolism;Gene Expression Profiling;Gene Expression Regulation - drug effects;Humans;Hydrogels - chemistry;Matrix stiffness;Mesenchymal Stem Cells - cytology;Mesenchymal Stem Cells - drug effects;Mesenchymal Stem Cells - metabolism;Mesenchymal stromal cells (MSCs);Microfluidics;Osteogenesis;Osteogenesis - drug effects;Osteogenesis - genetics;Polyacrylamide scaffold;Porosity;Scaffolds;Stiffness;Three dimensional;Tissue Scaffolds - chemistry;Two dimensional
日期:	2016-03-01
上傳時間:	2026-04-23 12:08:49 (UTC+8)
出版者:	Elsevier BV;England: Elsevier Ltd
摘要:	摘要： [Display omitted] Osteogenic potential of mesenchymal stromal cells (MSCs) is mechanosensitive. It’s affected by the mechanical properties of the cellular microenvironment, particularly its mechanical modulus. To explore the effect of mechanical modulus on osteogenesis in the third dimension (3D), this study used a novel polyacrylamide (PA) scaffold whose pores are monodisperse and spherical, the mechanical moduli of which can be tuned across a wide range. It was found that MSCs have similar proliferation rates in PA scaffolds independent of the matrix stiffness. The contractile force exerted by MSCs inside PA scaffolds was strong enough to deform the pores of scaffolds made of more compliant PAs (whose shear modulus, Gscaffold′<4kPa). Only scaffolds of the highest stiffness (Gscaffold′=12kPa) can withhold the contraction from MSCs. After osteogenic induction for 21days, the expression profiles of marker genes showed that PA scaffolds of Gscaffold′=12kPa promoted osteogenesis of MSCs. Confocal image analysis demonstrated that there are more F-actin cytoskeletons and bundled stress fibers at higher matrix moduli in 2D and 3D. Moreover, the 3D porous structure promotes osteogenesis of MSCs more than 2D flat substrates. Together, the differences of cellular behaviors when cultured in 2D and 3D systems are evident. The PA scaffolds developed in the present study can be used for further investigation into the mechanism of MSC mechanosensing in the 3D context. Mechanical properties of the microenvironment affect cellular behaviors, such as matrix stiffness. Traditionally, cell biological investigations have mostly employed cells growing on 2D substrates. The 3D porous PA scaffolds with the same topological conformation and pore sizes but different stiffness generated in this study showed that the differences of cellular behaviors in 2D and 3D systems are evident. Our 3D scaffolds provide insights into tissue engineering when stem cells incorporated with 3D scaffolds and support the future studies of cellular mechanobiology as well as the elucidation the role mechanical factor plays on the physiology and fate determination of MSCs in the 3D context. 其他題名： Acta Biomater 出版者： England: Elsevier Ltd 出版日期： 2016-03-01 出處： Acta biomaterialia, 2016-03, Vol.32, p.210-222 版權： 2016 Acta Materialia Inc. 版權： Copyright © 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. 識別號： ISSN: 1742-7061 識別號： EISSN: 1878-7568 識別號： DOI: 10.1016/j.actbio.2016.01.010 識別號： PMID: 26790775
顯示於類別:	[物理學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	16	檢視/開啟

在NCUIR中所有的資料項目都受到原著作權保護.

社群 sharing

資料載入中.....