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| 題名: | Microtubule C-Terminal Tails Can Change Characteristics of Motor Force Production |
| 作者: | 田溶根;Shojania Feizabadi, Mitra;Janakaloti Narayanareddy, Babu Reddy;Vadpey, Omid;Jun, Yonggun;Chapman, Dail;Rosenfeld, Steven;Gross, Steven P. |
| 貢獻者: | 理學院物理學系 |
| 關鍵詞: | Animals;Cancer;Cattle;Cell Line, Tumor;C‐terminal;Eg5;force production;Humans;Kinesin - metabolism;kinesin‐1;Kinetics;MCF-7 Cells;microtubule;Microtubules - metabolism;Protein Transport - physiology;Sports injuries;Tubulin - metabolism |
| 日期: | 2015-10-01 |
| 上傳時間: | 2026-04-23 12:30:25 (UTC+8) |
| 出版者: | Blackwell Munksgaard;Former Munksgaard: John Wiley & Sons A/S |
| 摘要: | 摘要： Control of intracellular transport is poorly understood, and functional ramifications of tubulin isoform differences between cell types are mostly unexplored. Motors' force production and detachment kinetics are critical for their group function, but how microtubule (MT) details affect these properties – if at all – is unknown. We investigated these questions using both a vesicular transport human kinesin, kinesin‐1, and also a mitotic kinesin likely optimized for group function, kinesin‐5, moving along either bovine brain or MCF7(breast cancer) MTs. We found that kinesin‐1 functioned similarly on the two sets of MTs – in particular, its mean force production was approximately the same, though due to its previously reported decreased processivity, the mean duration of kinesin‐1 force production was slightly decreased on MCF7 MTs. In contrast, kinesin‐5's function changed dramatically on MCF7 MTs: its average detachment force was reduced and its force–velocity curve was different. In spite of the reduced detachment force, the force–velocity alteration surprisingly improved high‐load group function for kinesin‐5 on the cancer‐cell MTs, potentially contributing to functions such as spindle‐mediated chromosome separation. Significant differences were previously reported for C‐terminal tubulin tails in MCF7 versus bovine brain tubulin. Consistent with this difference being functionally important, elimination of the tails made transport along the two sets of MTs similar. There is significant naturally occurring tubulin diversity, but the functional ramifications of such differences are predominantly unexplored, especially with regard to force production properties of the motors. The mean unloaded travel distances of both kinesin‐1 and Eg5 are decreased on MCF7 (cancer cell) microtubules relative to travel on bovine brain microtubules. This reduction might suggest impaired overall transport, but to our surprise the MCF7 microtubules change Eg5 function a way that improves multiple‐motor function.
其他題名： Traffic
出版者： Former Munksgaard: John Wiley & Sons A/S
出版日期： 2015-10
出處： Traffic (Copenhagen, Denmark), 2015-10, Vol.16 (10), p.1075-1087
資源來源： Wiley Online Library - AutoHoldings Journals
版權： 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
版權： 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
識別號： ISSN: 1398-9219
識別號： ISSN: 1600-0854
識別號： EISSN: 1600-0854
識別號： DOI: 10.1111/tra.12307
識別號： PMID: 26094820 |
| 顯示於類別: | [物理學系] 期刊論文
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