Microtubule C-Terminal Tails Can Change Characteristics of Motor Force Production

NCU Institutional Repository > 理學院 > 物理學系 > 期刊論文 > Item 987654321/105473

請使用永久網址來引用或連結此文件: https://ir.lib.ncu.edu.tw/handle/987654321/105473

題名:	Microtubule C-Terminal Tails Can Change Characteristics of Motor Force Production
作者:	田溶根;Shojania Feizabadi, Mitra;Janakaloti Narayanareddy, Babu Reddy;Vadpey, Omid;Jun, Yonggun;Chapman, Dail;Rosenfeld, Steven;Gross, Steven P.
貢獻者:	理學院物理學系
關鍵詞:	Animals;Cancer;Cattle;Cell Line, Tumor;C‐terminal;Eg5;force production;Humans;Kinesin - metabolism;kinesin‐1;Kinetics;MCF-7 Cells;microtubule;Microtubules - metabolism;Protein Transport - physiology;Sports injuries;Tubulin - metabolism
日期:	2015-10-01
上傳時間:	2026-04-23 12:30:25 (UTC+8)
出版者:	Blackwell Munksgaard;Former Munksgaard: John Wiley & Sons A/S
摘要:	摘要： Control of intracellular transport is poorly understood, and functional ramifications of tubulin isoform differences between cell types are mostly unexplored. Motors' force production and detachment kinetics are critical for their group function, but how microtubule (MT) details affect these properties – if at all – is unknown. We investigated these questions using both a vesicular transport human kinesin, kinesin‐1, and also a mitotic kinesin likely optimized for group function, kinesin‐5, moving along either bovine brain or MCF7(breast cancer) MTs. We found that kinesin‐1 functioned similarly on the two sets of MTs – in particular, its mean force production was approximately the same, though due to its previously reported decreased processivity, the mean duration of kinesin‐1 force production was slightly decreased on MCF7 MTs. In contrast, kinesin‐5's function changed dramatically on MCF7 MTs: its average detachment force was reduced and its force–velocity curve was different. In spite of the reduced detachment force, the force–velocity alteration surprisingly improved high‐load group function for kinesin‐5 on the cancer‐cell MTs, potentially contributing to functions such as spindle‐mediated chromosome separation. Significant differences were previously reported for C‐terminal tubulin tails in MCF7 versus bovine brain tubulin. Consistent with this difference being functionally important, elimination of the tails made transport along the two sets of MTs similar. There is significant naturally occurring tubulin diversity, but the functional ramifications of such differences are predominantly unexplored, especially with regard to force production properties of the motors. The mean unloaded travel distances of both kinesin‐1 and Eg5 are decreased on MCF7 (cancer cell) microtubules relative to travel on bovine brain microtubules. This reduction might suggest impaired overall transport, but to our surprise the MCF7 microtubules change Eg5 function a way that improves multiple‐motor function. 其他題名： Traffic 出版者： Former Munksgaard: John Wiley & Sons A/S 出版日期： 2015-10 出處： Traffic (Copenhagen, Denmark), 2015-10, Vol.16 (10), p.1075-1087 資源來源： Wiley Online Library - AutoHoldings Journals 版權： 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd 版權： 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. 識別號： ISSN: 1398-9219 識別號： ISSN: 1600-0854 識別號： EISSN: 1600-0854 識別號： DOI: 10.1111/tra.12307 識別號： PMID: 26094820
顯示於類別:	[物理學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	19	檢視/開啟

在NCUIR中所有的資料項目都受到原著作權保護.

社群 sharing

資料載入中.....