Alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora arose from duplication of a dual-functional predecessor of mitochondrial origin

Please use this identifier to cite or link to this item: https://ir.lib.ncu.edu.tw/handle/987654321/105708

Title:	Alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora arose from duplication of a dual-functional predecessor of mitochondrial origin
Authors:	曾議寬;Chang, Chia-Pei;Tseng, Yi-Kuan;Ko, Chou-Yuan;Wang, Chien-Chia
Contributors:	理學院統計研究所
Keywords:	Alanine-tRNA Ligase - genetics;Alanine-tRNA Ligase - metabolism;Amino Acid Sequence;Base Pairing;Base Sequence;Cell Nucleus - genetics;Codon, Initiator;Evolution, Molecular;Fungal Proteins - genetics;Fungal Proteins - metabolism;Gene Duplication;Genes, Fungal;Genes, Mitochondrial;Mitochondrial Proteins - genetics;Mitochondrial Proteins - metabolism;Molecular Biology;Molecular Sequence Data;RNA, Transfer, Ala - chemistry;Saccharomycetales - enzymology;Saccharomycetales - genetics
Date:	2012-01-01
Issue Date:	2026-04-23 12:48:38 (UTC+8)
Publisher:	Oxford University Press;England: Oxford University Press
Abstract:	摘要： In eukaryotes, the cytoplasmic and mitochondrial forms of a given aminoacyl-tRNA synthetase (aaRS) are typically encoded by two orthologous nuclear genes, one of eukaryotic origin and the other of mitochondrial origin. We herein report a novel scenario of aaRS evolution in yeast. While all other yeast species studied possess a single nuclear gene encoding both forms of alanyl-tRNA synthetase (AlaRS), Vanderwaltozyma polyspora, a yeast species descended from the same whole-genome duplication event as Saccharomyces cerevisiae, contains two distinct nuclear AlaRS genes, one specifying the cytoplasmic form and the other its mitochondrial counterpart. The protein sequences of these two isoforms are very similar to each other. The isoforms are actively expressed in vivo and are exclusively localized in their respective cellular compartments. Despite the presence of a promising AUG initiator candidate, the gene encoding the mitochondrial form is actually initiated from upstream non-AUG codons. A phylogenetic analysis further revealed that all yeast AlaRS genes, including those in V. polyspora, are of mitochondrial origin. These findings underscore the possibility that contemporary AlaRS genes in V. polyspora arose relatively recently from duplication of a dual-functional predecessor of mitochondrial origin. 其他題名： Nucleic Acids Res 出版者： England: Oxford University Press 出版日期： 2012-01-01 出處： Nucleic acids research, 2012-01, Vol.40 (1), p.314-322 資源來源： Oxford Journals Open Access Collection 版權： The Author(s) 2011. Published by Oxford University Press. 2011 識別號： ISSN: 0305-1048 識別號： ISSN: 1362-4962 識別號： ISSN: 1362-4954 識別號： EISSN: 1362-4962 識別號： DOI: 10.1093/nar/gkr724 識別號： PMID: 21908394
Appears in Collections:	[Graduate Institute of Statistics] journal & Dissertation

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