Impact of uremic environment on peritoneum: A proteomic view

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题名:	Impact of uremic environment on peritoneum: A proteomic view
作者:	吳忻怡;Wang, Hsien-Yi;Lin, Ching-Yih;Chien, Chih-Chiang;Kan, Wei-Chih;Tian, Yu-Feng;Liao, Pao-Chi;Wu, Hsin-Yi;Su, Shih-Bin
贡献者:	總教學中心通識教育中心
关键词:	2-DE;apolipoprotein A-I;apoptosis;Ascitic Fluid;Ascitic Fluid - metabolism;Biological and medical sciences;biomarkers;Biomarkers - metabolism;dialysis;Diverse techniques;drugs;Female;Fundamental and applied biological sciences. Psychology;Gallstones;Gallstones - metabolism;Gallstones - surgery;glycoproteins;Humans;inflammation;Male;Mass spectrometry;Medical sciences;metabolism;methods;Molecular and cellular biology;Nephrology. Urinary tract diseases;Nephropathies. Renovascular diseases. Renal failure;patients;Peritoneal Dialysis;Peritoneal fluid;peritoneum;permeability;Proteome;Proteome - metabolism;proteomics;Proteomics - methods;Renal failure;renal function;surgery;therapy;toxins;two-dimensional gel electrophoresis;Uremia;Uremia - metabolism;Uremia - therapy;Western blotting
日期:	2012-04-03
上传时间:	2026-04-23 12:53:03 (UTC+8)
出版者:	Elsevier;Kidlington: Elsevier B.V
摘要:	摘要： Peritoneal morphology and function are abnormal in uremia patients, but the contributing mechanisms are unclear. Here we attempted to characterize the protein targets that may be related to peritoneal change in patients with uremia and have not exposed to peritoneal dialysis fluid. Protein profiles of peritoneal fluids collected from patients with uremia and patients with normal renal function receiving laparoscopic cholecystectomy were displayed by two-dimensional gel electrophoresis (2-DE). Altered protein spots were excised and subjected to tryptic digestion followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Sixteen 2-DE protein spots were altered between two groups. Western blots confirmed that kininogen-1, apoptosis inhibitor 2, cat eye syndrome critical region protein 1, and apolipoprotein A-I had higher expression levels in the uremia samples. In contrast, synaptic vesicle 2-related protein, glial fibrillary acidic protein, and envelope glycoprotein (C2–V5 region) showed lower levels. The increased expression may result from a change in the permeability of the peritoneal membrane to middle-sized proteins or peritoneal inflammation with proteins sloughing off. All the identified proteins may provide a novel understanding of peritoneal changes caused by uremic toxins and may function as biomarkers or drug targets. [Display omitted] ► A first comparative proteomic analysis of uremic and normal peritoneal fluids. ► Aberrant level of 12 proteins in uremic peritoneal fluids. ► Identified proteins may shed light on studying of peritoneal damage. 其他題名： J Proteomics 出版者： Kidlington: Elsevier B.V 出版日期： 2012-04-03 出處： Journal of proteomics, 2012-04, Vol.75 (7), p.2053-2063 版權： 2012 Elsevier B.V. 版權： 2015 INIST-CNRS 版權： Copyright Â© 2012 Elsevier B.V. All rights reserved. 識別號： ISSN: 1874-3919 識別號： ISSN: 1876-7737 識別號： EISSN: 1876-7737 識別號： DOI: 10.1016/j.jprot.2012.01.011 識別號： PMID: 22266485
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