The change of microbial community from chlorinated solvent-contaminated groundwater after biostimulation using the metagenome analysis

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题名:	The change of microbial community from chlorinated solvent-contaminated groundwater after biostimulation using the metagenome analysis
作者:	曾議寬;Kao, Chih-Ming;Liao, Hung-Yu;Chien, Chih-Ching;Tseng, Yi-Kuan;Tang, Petrus;Lin, Chih-En;Chen, Ssu-Ching
贡献者:	理學院統計研究所
关键词:	bacteria;bacterial communities;biodegradation;Biodegradation, Environmental;Dechlorination;Dehalococcoides;Dehalococcoides mccartyi;DNA;ethylene;genes;groundwater;Groundwater - microbiology;Metagenome;Metagenomics;Microbial community;Microbiota - drug effects;molasses;Pseudomonas;ribosomal RNA;surfactants;Tetrachloroethane;Trichloroethene;Trichloroethylene - toxicity;vegetable oil
日期:	2016-01-25
上传时间:	2026-04-23 13:01:03 (UTC+8)
出版者:	Elsevier;Netherlands: Elsevier B.V
摘要:	摘要： •Matagenome analysis of PCE/TCE-contaminated groundwater.•Bacterial community of PCE/TCE-contaminated site using RDP and LCA methods was assessed.•SPRS caused the variation of bacterial community.•The detection of some genes related to TCE degradation pathway using KEGG analysis. The compositions of bacterial community in one site contaminated with PCE/TCE after the slow polycolloid-releasing substrate (SPRS) (contained vegetable oil, cane molasses, and surfactants) addition were analyzed. Results show that SPRS caused a rapid enhancement of reductive dechlorination of TCE. The transformation of PCE/TCE into ethene was observed after 20 days of operation. To compare the change of bacterial communities before and after SPRS addition, 16S rRNA amplicon sequencing using the metagenome analysis was performed. Results demonstrated the detection of the increased amounts of Dehalogenimonas by 2.2-fold, Pseudomonas by 3.4-fold and Sulfuricurvum by 4-fold with the analysis of the ribosomal database project (RDP). Metagenomic DNA was extracted from PCE/TCE-contaminated groundwater after SPRS addition, and subjected to sequencing. Results obtained from metagenomic sequencing indicate that genes from Dehalococcoides mccartyi was ranked as the second abundant bacteria among all of the detected bacteria via the analysis of the lowest common ancestor (LCA). Abundance of these bacterial groups, as shown above suggests their role in TCE biodegradation. Functional analysis of the metagenome, with the specific reference to chloroalkane and chloroalkene degradation, revealed the presence of some genes responsible for TCE biodegradation. Overall, results of this study provided new insights for a better understanding of the potential of biostimulation on TCE-contaminated sites. 其他題名： J Hazard Mater 出版者： Netherlands: Elsevier B.V 出版日期： 2016-01-25 出處： Journal of hazardous materials, 2016-01, Vol.302, p.144-150 版權： 2015 Elsevier B.V. 版權： Copyright © 2015 Elsevier B.V. All rights reserved. 識別號： ISSN: 0304-3894 識別號： EISSN: 1873-3336 識別號： DOI: 10.1016/j.jhazmat.2015.09.047 識別號： PMID: 26474376
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