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https://ir.lib.ncu.edu.tw/handle/987654321/107029
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| 題名: | Development of a portable impedance detection system for monitoring the growth of mouse L929 cells |
| 作者: | 蔡章仁;Chen, Ching Jung;Liu, Jen Tsai;Chang, Shwu-Jen;Lee, Ming-Wei;Tsai, Jang-Zern |
| 貢獻者: | 資訊電機學院電機工程學系 |
| 關鍵詞: | Impedance measurement;Interdigitating electrodes;L929 cells;Microfabrication |
| 日期: | 2012-09-01 |
| 上傳時間: | 2026-04-23 13:53:44 (UTC+8) |
| 出版者: | Taiwan Institute of Chemical Engineers;Elsevier B.V |
| 摘要: | 摘要： ► We made a low-cost portable impedance measurement system for cell growth monitoring. ► AC impedance measurement was conducted with interdigitating microelectrodes. ► Capacitive impedance change is attributed to cell proliferation and metabolites. ► Measured impedance changes manifest lag, log, and stationary phases of cell growth. A portable impedance measurement system was used to monitor the growth of L929 cells. The interdigitating microelectrodes of our portable system were produced by microfabrication technology with a width of 8μm, a gap of 8μm, and a length of 500μm. These microelectrodes provided a high electric field for AC impedance measurements. When mouse L929 cells were cultured on the electrode surface and pre-coated with poly-l-lysine, the changes in the impedance on the interdigitating electrodes represented the lag, log, and stationary phases of a typical cell growth curve. The impedance measurement result was fitted to an equivalent circuit, which showed that the capacitance signal was the major contributor to the total impedance of the cell culture. The capacitance arises from the cell capacitance, the double-layer capacitance and the capacitance of the poly-l-lysine-coated surface, and the changes in capacitance were probably due to cell proliferation and the resulting changes in cell metabolites. To make the device portable and provide rapid results, we designed a novel impedance detection system comprised of a microprocessor, an AD5933 impedance converter (IC), and the interdigitating microelectrodes. This device was successfully used to observe the changes in the metabolism and proliferation of L929 cells at different stages of cell growth with low cell concentrations. Further, the portable impedance measurement device is a useful analytical tool for monitoring cell growth. It will integrate easily with wireless circuits for applications in aseptic cell biology.
出版者： Elsevier B.V
出版日期： 2012-09
出處： Journal of the Taiwan Institute of Chemical Engineers, 2012-09, Vol.43 (5), p.678-684
資源來源： Elsevier ScienceDirect Journals Complete
版權： 2012 Taiwan Institute of Chemical Engineers
識別號： ISSN: 1876-1070
識別號： EISSN: 1876-1089
識別號： DOI: 10.1016/j.jtice.2012.04.008 |
| 顯示於類別: | [電機工程學系] 期刊論文
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