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    題名: Impedance sensor for rapid enumeration of E. coli in milk samples
    作者: 蔡章仁;Liu, Jen-Tsai;Settu, Kalpana;Tsai, Jang-Zern;Chen, Ching-Jung
    貢獻者: 資訊電機學院電機工程學系
    關鍵詞: Electrochemical impedance spectroscopy;Escherichia coli;Interdigitated microelectrode;Milk
    日期: 2015-11-10
    上傳時間: 2026-04-23 14:14:10 (UTC+8)
    出版者: Elsevier Ltd.;Elsevier Ltd
    摘要: 摘要: Detection of pathogenic bacteria in dairy farms and processing plants is important for milk quality control. In this study, a simple in situ method for determining the bacterial concentration in milk was developed with an interdigitated microelectrode sensor. This method was based on a punctual measurement of the electrode–milk interface impedance change due to bacterial metabolism in the milk. The interface impedance varied predominately at low frequencies, attributed to the change on the double-layer capacitance. The detection time TD, defined as the time required to attain a −10% impedance change at 10Hz, became a practicable means for the estimation of initial E. coli concentration in milk samples. Regression analysis resulted in a third-order polynomial relationship between the detection time and the logarithm of the initial E. coli concentration (N0, cells/ml) in a milk sample, i.e., log N0=11.48–2.33 TD+0.288(TD)2–0.0154(TD)3 with R2=0.9988. The sensor was able to detect E. coli in milk of initial concentrations as low as 7cells/ml. The proposed impedance sensor is suitable for determining bacterial contamination at dairy farms and processing plants.
    出版者: Elsevier Ltd
    出版日期: 2015-11-10
    出處: Electrochimica acta, 2015-11, Vol.182, p.89-95
    版權: 2015 Elsevier Ltd
    識別號: ISSN: 0013-4686
    識別號: EISSN: 1873-3859
    識別號: DOI: 10.1016/j.electacta.2015.09.029
    顯示於類別:[電機工程學系] 期刊論文

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